The Second School of Clinical Medicine, Southern Medical University, Guangzhou, China.
Department of General Surgery, The First Medical Center of Chinese PLA General Hospital, Beijing, China.
J Clin Lab Anal. 2023 Feb;37(3):e24797. doi: 10.1002/jcla.24797. Epub 2023 Feb 1.
IL-8 has been implicated in the malignant progression of various types of cancers; however, the precise molecular mechanisms associated with IL-8 in breast cancer (BRCA) are unclear.
We analyzed the clinical signature and immune characteristics of BRCA patients and its correlation with IL-8 expression using The Cancer Genome Atlas (TCGA) datasets. The role of IL-8 in epithelial-mesenchymal transition (EMT) was verified through Western blotting, Cell Counting Kit-8 assay, and wound healing assays, as well as cell invasion experiments.
Through a comprehensive bioinformatics study, we determined that high IL-8 expression was associated with poor prognosis. Enrichment analysis revealed that high IL-8 expression was enriched in immune-related processes and cancer-related signaling pathways. In addition, IL-8 was associated with most of the immune-infiltrating cells, and high IL-8 expression indicated poor response to immunotherapy. Importantly, we found that IL-8 induced EMT in vitro.
Taken together, our data indicate that IL-8 may be a potential and valuable prognostic marker in BRCA, which may induce adverse outcomes by modulating the immune response and promoting EMT in BRCA patients.
白细胞介素 8（IL-8）已被牵涉到多种类型癌症的恶性进展中；然而，与乳腺癌（BRCA）中 IL-8 相关的确切分子机制尚不清楚。
我们使用癌症基因组图谱（TCGA）数据集分析了 BRCA 患者的临床特征和免疫特征及其与 IL-8 表达的相关性。通过 Western blot、细胞计数试剂盒-8 检测、划痕愈合实验和细胞侵袭实验验证了 IL-8 在上皮-间充质转化（EMT）中的作用。
通过全面的生物信息学研究，我们确定高 IL-8 表达与预后不良有关。富集分析显示，高 IL-8 表达富集在免疫相关过程和癌症相关信号通路中。此外，IL-8 与大多数免疫细胞浸润有关，高 IL-8 表达表明对免疫治疗的反应不佳。重要的是，我们发现 IL-8 在体外诱导 EMT。
综上所述，我们的数据表明，IL-8 可能是 BRCA 中一个有潜力和有价值的预后标志物，它可能通过调节免疫反应和促进 BRCA 患者的 EMT 来诱导不良后果。
