Department of Bioengineering and Technology, Gauhati University, Gawahati, Assam, India.
Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.
J Gastrointest Cancer. 2024 Sep;55(3):1239-1255. doi: 10.1007/s12029-024-01063-1. Epub 2024 Jun 23.
Interleukin-8 (IL8), Interleukin-12 (IL12) and Interleukin-13 (IL13) are cytokines that play regulatory role in cancer pathogenesis. We analysed their expression profile to evaluate as molecular biomarkers of esophageal squamous cell carcinoma (ESCC) and their association with different parameters and patient survival.
Expression analysis was performed by Real time quantitative polymerase chain reaction and receiver operating characteristic (ROC) curve analysis was done. The expression profiles were associated with different clinicopathological and dietary factors. Survival and hazard analysis were also performed.
IL8 expression showed upregulation in tissue (p = 0.000) and blood samples (p = 0.481), IL12 expression showed downregulation in tissue samples (p = 0.064) and upregulation in blood samples (p = 0.689) and IL13 expression showed upregulation in tissue (p = 0.000) and blood samples (p = 0.006). IL13 expression in tissue showed the highest area under the curve (AUC) value (0.773) for ESCC diagnosis, followed by IL8 expression in tissue (0.704) and IL13 expression in blood (0.643). This study also reveals the correlation of studied cytokines in tissue and blood level. Different clinicopathological and dietary factors showed significant association (p < 0.05) with IL8, IL12 and IL13 expression and with survival of ESCC patients. IL8 expression in blood and IL12 expression in tissue and blood showed significant association (p < 0.05) with patient survival.
Altered expression of IL8, IL12 and IL13 may be associated with ESCC progression. Overexpression of IL8 and IL13 in tissue samples may be potential biomarkers for ESCC screening. Additionally, both survival and hazard analysis data indicate the effects of different parameters on the prognosis of ESCC patients.
白细胞介素-8（IL-8）、白细胞介素-12（IL-12）和白细胞介素-13（IL-13）是在癌症发病机制中起调节作用的细胞因子。我们分析了它们的表达谱，以评估其作为食管鳞状细胞癌（ESCC）的分子生物标志物及其与不同参数和患者生存的关系。
通过实时定量聚合酶链反应进行表达分析，并进行接收者操作特征（ROC）曲线分析。表达谱与不同的临床病理和饮食因素相关联。还进行了生存和危险分析。
IL-8 在组织（p=0.000）和血液样本（p=0.481）中的表达上调，IL-12 在组织样本中的表达下调（p=0.064）和血液样本中的表达上调（p=0.689），IL-13 在组织（p=0.000）和血液样本（p=0.006）中的表达上调。组织中 IL-13 的表达对 ESCC 诊断具有最高的曲线下面积（AUC）值（0.773），其次是组织中 IL-8 的表达（0.704）和血液中 IL-13 的表达（0.643）。这项研究还揭示了组织和血液水平中研究细胞因子的相关性。不同的临床病理和饮食因素与 IL-8、IL-12 和 IL-13 的表达以及 ESCC 患者的生存有显著相关性（p<0.05）。血液中 IL-8 的表达和组织及血液中 IL-12 的表达与患者的生存有显著相关性（p<0.05）。
IL-8、IL-12 和 IL-13 的表达改变可能与 ESCC 的进展有关。组织样本中 IL-8 和 IL-13 的过表达可能是 ESCC 筛查的潜在生物标志物。此外，生存和危险分析数据均表明不同参数对 ESCC 患者预后的影响。
