一线抗风湿药物对 T 细胞活化的抗炎作用。
Anti-inflammatory Effects of First-line Anti-arthritic Drugs on T-cell Activation.
机构信息
Department of Rheumatology, Faculty of Medicine and Health, The University of Sydney, Sydney, Australia.
Department of Rheumatology, Westmead Hospital, Sydney, 2145, Australia.
出版信息
Antiinflamm Antiallergy Agents Med Chem. 2023;21(3):188-193. doi: 10.2174/1871523022666230201144553.
AIM
The in vitro effects of commonly used first-line anti-arthritic drugs on early stages of T-cell activation were examined.
METHODS
The 2B4.11 murine T cell hybridoma cell line recognizing pigeon cytochrome c (PCC) as the antigen was co-cultured with the histocompatible antigen presenting B cell hybridoma line LK35.2, PCC, and anti-arthritic drugs, including methotrexate, hydroxychloroquine, salazopyrine, cyclosporin, and leflunomide. After 16 hours of incubation, the supernatant was removed, and cytokines were assayed.
RESULTS
Anti-arthritic drugs inhibited the production of pro-inflammatory cytokines IL-2, IL-6, IFN-γ, GM-CSF, and TNF-α (Th1 cytokines) to a varying extent. Surprisingly, leflunomide, salazopyrine, prednisone and indomethacin as well as blocking Th1 cytokines, stimulated the production of the anti-inflammatory cytokine IL-10, a Th2 cytokine.
CONCLUSION
Anti-arthritic medications can inhibit the production of pro-inflammatory cytokines and in some cases, incite a Th2 response that could potentially inhibit the progression of the immune response.
目的
研究常用一线抗风湿药物对 T 细胞活化早期阶段的体外影响。
方法
用识别 pigeon cytochrome c（PCC）作为抗原的 2B4.11 鼠 T 细胞杂交瘤细胞系与同种相容的抗原呈递 B 细胞杂交瘤系 LK35.2、PCC 和抗风湿药物（包括甲氨蝶呤、羟氯喹、柳氮磺胺吡啶、环孢素和来氟米特）共同培养。孵育 16 小时后，去除上清液并检测细胞因子。
结果
抗风湿药物不同程度地抑制了促炎细胞因子 IL-2、IL-6、IFN-γ、GM-CSF 和 TNF-α（Th1 细胞因子）的产生。令人惊讶的是，来氟米特、柳氮磺胺吡啶、泼尼松和吲哚美辛以及阻断 Th1 细胞因子的药物，刺激了抗炎细胞因子 IL-10（Th2 细胞因子）的产生。
结论
抗风湿药物可以抑制促炎细胞因子的产生，在某些情况下，还会引发 Th2 反应，从而可能抑制免疫反应的进展。
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