Sharjah Institute for Medical Research, University of Sharjah, P.O. Box 27272, Sharjah, United Arab Emirates.
Department of Medicinal Chemistry, College of Pharmacy, University of Sharjah, P.O. Box 27272, Sharjah, United Arab Emirates.
Sci Rep. 2023 Feb 1;13(1):1870. doi: 10.1038/s41598-023-27958-w.
The use of alternative tobacco products, particularly medwakh, has expanded among youth in the Middle East and around the world. The present study is conducted to investigate the biochemical and pathophysiological changes caused by medwakh smoking, and to examine the salivary metabolomics profile of medwakh smokers. Saliva samples were collected from 30 non-smokers and 30 medwakh smokers and subjected to metabolomic analysis by UHPLC-ESI-QTOF-MS. The CRP and Glutathione Peroxidase 1 activity levels in the study samples were quantified by ELISA and the total antioxidant capacity (TAC) by TAC assay kits. Statistical measurements and thorough validation of data obtained from untargeted metabolomics identified 37 uniquely and differentially abundant metabolites in saliva of medwakh smokers. The levels of phthalate, L-sorbose, cytosine, uridine, alpha-hydroxy hippurate, and L-nicotine were noticeably high in medwakh smokers. Likewise, 20 metabolic pathways were differentially altered in medwakh smokers. This study identified a distinctive saliva metabolomics profile in medwakh smokers associated with altered redox homeostasis, metabolic pathways, antioxidant system, and CRP levels. The impact of the altered metabolites in medwakh smokers and their diagnostic utility require further research in large cohorts.
另类烟草制品，尤其是水烟，在中东和世界各地的青少年中使用有所增加。本研究旨在调查水烟吸烟引起的生化和病理生理变化，并研究水烟吸烟者的唾液代谢组学特征。采集了 30 名非吸烟者和 30 名水烟吸烟者的唾液样本，通过 UHPLC-ESI-QTOF-MS 进行代谢组学分析。通过 ELISA 定量测定研究样本中的 CRP 和谷胱甘肽过氧化物酶 1 活性水平，通过 TAC 测定试剂盒测定总抗氧化能力 (TAC)。非靶向代谢组学的统计测量和对数据的深入验证，确定了水烟吸烟者唾液中 37 种独特的、差异丰富的代谢物。水烟吸烟者唾液中的邻苯二甲酸酯、L-山梨糖、胞嘧啶、尿嘧啶、α-羟基马尿酸和 L-尼古丁水平明显较高。同样，20 种代谢途径在水烟吸烟者中发生了差异改变。这项研究在水烟吸烟者中确定了一个独特的唾液代谢组学特征，与氧化还原平衡、代谢途径、抗氧化系统和 CRP 水平的改变有关。需要在大样本中进一步研究水烟吸烟者中改变的代谢物及其诊断效用。
