Lanzhou University, Lanzhou, 730000, Gansu, China.
Lanzhou University First Affiliated Hospital, Lanzhou, 730030, Gansu, China.
Reprod Biol Endocrinol. 2023 Feb 1;21(1):16. doi: 10.1186/s12958-023-01056-y.
Owing to its difficulty in degrading and ease of accumulation in the body, perfluorooctanoic acid (PFOA) has a detrimental effect on reproduction. This study aimed to examine the effect of PFOA concentration in follicular fluid during ovulation stimulation on embryo quality and the impact of PFOA exposure on the metabolic components of follicular fluid. This was a single-center prospective study that included 25 patients with diminished ovarian reserve (DOR), 25 with normal ovarian reserve (NOR), and 25 with polycystic ovary syndrome (PCOS). Follicular fluid samples were analyzed using ultra-high performance liquid chromatography-tandem mass spectrometry. We demonstrated that the PFOA levels of follicular fluid in the DOR group were higher than those in the NOR group and PCOS group (P < 0.05). PFOA concentration in the PCOS group was negatively correlated with high-quality embryos (P < 0.05). To gain more insight into the impact of PFOA on the metabolic composition of follicular fluid, we classified the DOR group based on the PFOA concentration, for which metabolomic analysis was performed. In the high-concentration PFOA group, there was an increase and a decrease in three and nine metabolites, respectively, compared to that in the low-concentration group. These results suggest that PFOA may alter the metabolic composition of follicular fluid, thus, affecting ovarian reserve function.
由于其难以降解和易于在体内积累，全氟辛酸（PFOA）对生殖系统有不良影响。本研究旨在研究排卵刺激过程中卵泡液中的 PFOA 浓度对胚胎质量的影响，以及 PFOA 暴露对卵泡液代谢成分的影响。这是一项单中心前瞻性研究，包括 25 名卵巢储备功能减退（DOR）患者、25 名卵巢储备功能正常（NOR）患者和 25 名多囊卵巢综合征（PCOS）患者。使用超高效液相色谱-串联质谱法分析卵泡液样本。我们发现，DOR 组卵泡液中的 PFOA 水平高于 NOR 组和 PCOS 组（P<0.05）。PCOS 组的 PFOA 浓度与优质胚胎呈负相关（P<0.05）。为了更深入地了解 PFOA 对卵泡液代谢成分的影响，我们根据 PFOA 浓度对 DOR 组进行了分类，并进行了代谢组学分析。在高浓度 PFOA 组中，与低浓度组相比，分别有三种和九种代谢物增加和减少。这些结果表明，PFOA 可能改变卵泡液的代谢组成，从而影响卵巢储备功能。
