Department of Biochemistry, Faculty of Sciences, University of Yaoundé I, Box 812, Yaoundé, Cameroon.
Department of Biological Sciences, Faculty of Sciences, University of Maroua, Box 814, Maroua, Cameroon.
Biomed Res Int. 2023 Jan 23;2023:2754725. doi: 10.1155/2023/2754725. eCollection 2023.
The present study was carried out to investigate the anti-inflammatory activity of a methanolic extract and fractions of stems. The crude extract was obtained by maceration of the powder in methanol and fractions by vacuum chromatography from the methanolic extract. To study the anti-inflammatory activity , red blood cell lysis inhibition assay and albumin denaturation inhibition were performed, while measurements of carrageenan-induced paw oedema and formalin-induced pain in albino mice were performed. Acute toxicity and cytotoxicity studies of the fraction F2 were performed, as well as its HPLC, and some biochemical parameters were quantified. crude extract (UCCE) at 250 and 500 g/mL completely inhibited albumin denaturation, while decreasing 75.5% of heat blood cell lysis at 500 g/mL. The fractions 128-136 (F3), 10-11 (F1), and 56-62 (F2) at 500 g/mL displayed a significant anti-inflammatory activity with percentages of inhibition of 60.5, 67.4, and 100%, respectively. Administration of fraction F2 (25, 50, and 100 mg/kg, p.o.) produced a dose-dependent inhibition of formalin-induced pain of 60.2% at 50 mg/kg in the neurogenic phase ( < 0.05) and 70.2% at 25 mg/kg in the inflammatory phase ( < 0.05). Similarly, the time-dependent increase in carrageenan-induced paw circumference induced by carrageenan was inhibited by pretreatment with F2: 50% of inhibition at 25 mg/kg after 30 min ( < 0.05) and 96.5% inhibition at 25 mg/kg after 6 h ( < 0.05). In this research, the fraction F2 presented its maximum analgesic property at 50 mg/kg, while it presented the highest anti-inflammatory property at 25 mg/kg. The oral lethal median dose (LD) of F2 was determined to be greater than 2000 mg/kg; further low cytotoxicity in RAW cells was also observed. Overall, this work shows that the methanolic crude extract and fractions, mainly F2, of stem have interesting anti-inflammatory properties.
本研究旨在探究茎的甲醇提取物及其馏分的抗炎活性。粗提取物通过将粉末在甲醇中浸渍获得，馏分则通过真空色谱法从甲醇提取物中获得。为了研究抗炎活性，进行了红细胞溶血抑制试验和白蛋白变性抑制试验，同时在白化小鼠中进行了角叉菜胶诱导的足肿胀和福马林诱导的疼痛测量。还进行了馏分 F2 的急性毒性和细胞毒性研究，以及其 HPLC，并定量了一些生化参数。在 250 和 500μg/ml 时，粗提取物（UCCE）完全抑制了白蛋白变性，而在 500μg/ml 时使热红细胞溶血降低了 75.5%。在 500μg/ml 时，馏分 128-136（F3）、10-11（F1）和 56-62（F2）显示出显著的抗炎活性，抑制率分别为 60.5%、67.4%和 100%。F2 馏分（25、50 和 100mg/kg，po）给药产生了剂量依赖性抑制福马林诱导的疼痛，在神经原性相（<0.05）中 50mg/kg 时为 60.2%，在炎症相（<0.05）中 25mg/kg 时为 70.2%。同样，F2 预处理抑制了角叉菜胶诱导的足肿胀的时间依赖性增加：在 30 分钟时，25mg/kg 时抑制了 50%（<0.05），在 6 小时时，25mg/kg 时抑制了 96.5%（<0.05）。在这项研究中，F2 馏分在 50mg/kg 时表现出最大的镇痛特性，而在 25mg/kg 时表现出最高的抗炎特性。F2 的口服半数致死剂量（LD）确定大于 2000mg/kg；还观察到其在 RAW 细胞中的低细胞毒性。总的来说，这项工作表明茎的甲醇粗提取物及其馏分，主要是 F2，具有有趣的抗炎特性。
