Huang Bowen, Ong Adabelle, Wang Wenli, He Yu, Xiao Yongjun
Key Laboratory for Analytical Science of Food Safety and Biology (Ministry of Education), College of Chemistry, Fuzhou University, University Town, 2 Xue Yuan Road, Fuzhou, 350108, Fujian, China.
International Food and Water Research Centre, Waters Pacific Pte Ltd, Science Park II, Singapore, 117528, Singapore.
Anal Bioanal Chem. 2023 Mar;415(8):1571-1581. doi: 10.1007/s00216-023-04560-7. Epub 2023 Feb 2.
The detection of emerging contaminants (ECs) and understanding their ecotoxicity has brought new challenges to water pollution control. Triclosan (TCS), as an emerging contaminant, is a commonly used antibacterial agent widely present in the environment. Microdialysis (MD), as a sampling technique, can overcome some of the deficiencies of traditional approaches to sampling, using sources such as blood, urine, tissue, and target organs, in terms of invasiveness, time from collection to analysis, and possible changes during sample preparation. In this study, we coupled MD with analysis using UHPLC-QTOF/MS to identify the endogenous metabolites in the liver as biomarkers of the exposure of living crucian carp to TCS. The identified biomarkers were then quantified using UHPLC-MS/MS to continuously monitor the effect of TCS on endogenous metabolites in the liver of living crucian carp, which contributes to a better understanding of the toxicological effect of TCS. The experimental results demonstrated that TCS exposure interfered with the metabolic pathways of amino acids (L-isoleucine and L-histidine), purines (xanthine and hypoxanthine), and small nerve molecules (acetylcholine and choline).
新兴污染物（ECs）的检测及其生态毒性的理解给水污染控制带来了新的挑战。三氯生（TCS）作为一种新兴污染物，是一种广泛存在于环境中的常用抗菌剂。微透析（MD）作为一种采样技术，在侵入性、从采集到分析的时间以及样品制备过程中可能发生的变化等方面，可以克服传统采样方法在使用血液、尿液、组织和靶器官等样本来源时的一些不足。在本研究中，我们将微透析与超高效液相色谱-四极杆飞行时间质谱联用分析，以鉴定鲫鱼肝脏中的内源性代谢物作为鲫鱼暴露于三氯生的生物标志物。然后使用超高效液相色谱-串联质谱对鉴定出的生物标志物进行定量，以持续监测三氯生对活体鲫鱼肝脏内源性代谢物的影响，这有助于更好地理解三氯生的毒理学效应。实验结果表明，三氯生暴露干扰了氨基酸（L-异亮氨酸和L-组氨酸）、嘌呤（黄嘌呤和次黄嘌呤）以及小分子神经递质（乙酰胆碱和胆碱）的代谢途径。
