Mathematical, Computational and Systems Biology (MCSB) Program, University of California, Irvine, Irvine, CA, USA.
Department of Dermatology, University of Michigan Medical School, Ann Arbor, MI, USA.
Cell Rep. 2023 Jan 31;42(1):111994. doi: 10.1016/j.celrep.2023.111994. Epub 2023 Jan 24.
Palmoplantar skin is structurally and functionally unique, but the transcriptional programs driving this specialization are unclear. Here, we use bulk and single-cell RNA sequencing of human palm, sole, and hip skin to describe the distinguishing characteristics of palmoplantar and non-palmoplantar skin while also uncovering differences between palmar and plantar sites. Our approach reveals an altered immune environment in palmoplantar skin, with downregulation of diverse immunological processes and decreased immune cell populations. Further, we identify specific fibroblast populations that appear to orchestrate key differences in cell-cell communication in palm, sole, and hip. Dedicated keratinocyte analysis highlights major differences in basal cell fraction among the three sites and demonstrates the existence of two spinous keratinocyte populations constituting parallel, site-selective epidermal differentiation trajectories. In summary, this deep characterization of highly adapted palmoplantar skin contributes key insights into the fundamental biology of human skin and provides a valuable data resource for further investigation.
手掌足底皮肤在结构和功能上具有独特性，但驱动这种特化的转录程序尚不清楚。在这里，我们使用人类手掌、足底和臀部皮肤的批量和单细胞 RNA 测序来描述手掌足底和非手掌足底皮肤的区别特征，同时揭示手掌和足底部位之间的差异。我们的方法揭示了手掌足底皮肤中免疫环境的改变，多种免疫过程下调和免疫细胞群体减少。此外，我们确定了特定的成纤维细胞群体，这些群体似乎在手掌、足底和臀部的细胞间通讯中起着关键作用。专门的角质形成细胞分析突出了三个部位之间基底细胞分数的主要差异，并证明了存在两种棘状角质形成细胞群体，构成了平行的、部位选择性的表皮分化轨迹。总之，对手掌足底高度适应皮肤的深入特征分析为人类皮肤的基础生物学提供了关键见解，并为进一步研究提供了有价值的数据集。
