中国小麦花叶病毒感染后小麦LRR-RLK家族基因的全基因组鉴定与分析

Genome-wide identification and analysis of wheat LRR-RLK family genes following Chinese wheat mosaic virus infection.

作者信息

Liu Shuang, Lei Jiajia, Zhang Juan, Liu Hanhong, Ye Zhuangxin, Yang Jin, Lu Qiseng, Liu Peng, Chen Jianping, Yang Jian

机构信息

College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China.

State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Virology, Ningbo University, Ningbo, China.

出版信息

Front Plant Sci. 2023 Jan 17;13:1109845. doi: 10.3389/fpls.2022.1109845. eCollection 2022.

BACKGROUND

As the largest plant receptor-like protein kinase (RLK) superfamily, the 21 leucine-rich repeat receptor-like kinases (LRR-RLKs) family are involved in plant 22 growth, development, and stress responses. However, the functions of LRR-RLKs in 23 wheat immunity remain unknown.

RESULTS

In the current study, 929 LRR-RLKs were identified in 25 genome database using the BLAST and hidden Markov models (HMM) approach and 26 divided into 14 clades. Chromosomal localization and synteny analysis revealed that 27 were randomly distributed on all chromosomes with 921 collinear 28 events. Through the cis-acting elements analysis, we observed that 29 participated in hormone response, light response, development, metabolism, and 30 response to environmental stress. The transcript level of 14 random selected 31 from each subfamily was regulated by plant hormone treatment and 32 Chinese wheat mosaic virus (CWMV) infection. The function of in 33 wheat resistance to CWMV infection was further investigated by virus-induced gene 34 silencing assay. Additionally, the accumulation of MeJA response genes, as well as 35 CWMV RNA were not changed in the silencing plants under MeJA 36 treatment.

CONCLUSIONS

Our results demonstrated that TaLRR-RLKs play an important role in 38 wheat resistance to viral infection hormone signals and lay the groundwork for the 39 functional study of TaLRR-RLKs in wheat.

背景

作为最大的植物类受体蛋白激酶（RLK）超家族，21个富含亮氨酸重复序列的类受体激酶（LRR-RLKs）家族参与植物生长、发育及胁迫响应。然而，LRR-RLKs在小麦免疫中的功能尚不清楚。

结果

在本研究中，利用BLAST和隐马尔可夫模型（HMM）方法在基因组数据库中鉴定出929个LRR-RLKs，并将其分为14个进化枝。染色体定位和共线性分析表明，它们随机分布于所有染色体上，共有921个共线性事件。通过顺式作用元件分析，我们观察到它们参与激素响应、光响应、发育、代谢及环境胁迫响应。从每个亚家族中随机选取的14个的转录水平受植物激素处理和中国小麦花叶病毒（CWMV）侵染的调控。通过病毒诱导的基因沉默试验进一步研究了其在小麦抗CWMV侵染中的功能。此外，在茉莉酸甲酯（MeJA）处理下，沉默植株中MeJA响应基因以及CWMV RNA的积累没有变化。