Department of Medicine, The George Washington University, 2300 I Street NW, Washington, DC 20037, USA.
The George Washington Cancer Center, The George Washington University, Science & Engineering Hall, Ste 8300, Washington, DC 20052, USA.
Nanomedicine (Lond). 2022 Dec;17(29):2159-2171. doi: 10.2217/nnm-2022-0311. Epub 2023 Feb 3.
To investigate Prussian blue nanoparticles (PBNPs) coated with the synthetic analog of dsRNA polyinosinic-polycytidylic acid (polyIC) for their ability to function as HIV latency reversing agents. A layer-by-layer method was used to synthesize polyIC-coated PBNPs (polyIC-PBNPs). PolyIC-PBNPs were stable and monodisperse, maintained the native absorbance properties of both polyIC and PBNPs and were obtained with high nanoparticle collection yield and polyIC attachment efficiencies. PolyIC-PBNPs were more effective in reactivating latent HIV than free polyIC in a cell model of HIV latency. Furthermore, polyIC-PBNPs were more effective in promoting immune activation than free polyIC in CD4 and CD8 T cells. PBNPs function as efficient carriers of nucleic acids to directly reverse HIV latency and enhance immune activation.
研究普鲁士蓝纳米颗粒（PBNPs）包裹人工合成双链 RNA 聚肌苷酸-聚胞苷酸（polyIC）类似物，以考察其作为 HIV 潜伏逆转剂的功能。采用层层自组装的方法合成聚肌苷酸包裹的 PBNPs（polyIC-PBNPs）。polyIC-PBNPs 稳定且单分散，保持了 polyIC 和 PBNPs 的固有吸收特性，并且具有高的纳米颗粒收集产率和 polyIC 附着效率。在 HIV 潜伏的细胞模型中，polyIC-PBNPs 比游离 polyIC 更有效地激活潜伏的 HIV。此外，polyIC-PBNPs 在促进 CD4 和 CD8 T 细胞的免疫激活方面比游离 polyIC 更有效。PBNPs 可作为核酸的有效载体，直接逆转 HIV 潜伏并增强免疫激活。
