Dong Kairui, Xu Yulin, Wang Qiong, Liu Xiuyan, Xue Junzeng, Wu Huixian
College of Marine Ecology and Environment, Shanghai Ocean University, Shanghai 201306, China; Centre for Research on the Ecological Security of Ports and Shipping, Shanghai Ocean University, Shanghai 201306, China.
College of Meterial and Environmental engineering, Hangzhou Dianzi University, Hangzhou 310018, China.
Mar Pollut Bull. 2023 Mar;188:114652. doi: 10.1016/j.marpolbul.2023.114652. Epub 2023 Feb 1.
Effects of the membrane separation + N deoxidation process on the abundance and activity of bacteria were examined under two salinity conditions at Yangshan Port, Shanghai, China. Sequencing of 16S rRNA gene amplicons demonstrated a decrease in the diversity and activity of bacteria in fresh water and marine water, with a total removal rate of approximately 63 % and 69 %, respectively. Indicator bacteria decreased to 10 CFU·100 mL, which met the IMO D-2 standard. A total of 13 potential pathogens were detected after treatment, indicating that there is still a risk of pathogenic bacteria invasion in the discharge water, particularly marine bacteria, and that the D-2 standard may be insufficient as a preventive measure against pathogenic bacteria transfer. The results will provide reference for government supervision, and will also be important for monitoring foreign bacteria and technology development.
在中国上海洋山港的两种盐度条件下，研究了膜分离+N脱氧工艺对细菌丰度和活性的影响。16S rRNA基因扩增子测序表明，淡水和海水中细菌的多样性和活性均有所下降，总去除率分别约为63%和69%。指示菌降至10 CFU·100 mL，符合国际海事组织D-2标准。处理后共检测到13种潜在病原体，表明排放水中仍存在病原菌入侵风险，尤其是海洋细菌，且D-2标准作为预防病原菌转移的措施可能不够充分。研究结果将为政府监管提供参考，对于监测外来细菌和技术发展也具有重要意义。
