Shinde Vinod Ravasaheb, Khatun Sajmina, Thanekar Ajinkya Madhukar, Hak Arshadul, Rengan Aravind Kumar
Department of Biomedical Engineering, Indian Institute of Technology Hyderabad, Kandi, Telangana, India.
Department of Biomedical Engineering, Indian Institute of Technology Hyderabad, Kandi, Telangana, India.
Photodiagnosis Photodyn Ther. 2023 Mar;41:103314. doi: 10.1016/j.pdpdt.2023.103314. Epub 2023 Feb 2.
The synthesis of carbon dots using plant leaves is a facile and economically viable approach. Here we report the development of lipid-coated red fluorescent carbon dots (LRCDs), a biocompatible and stable nanomaterial, utilizing Clitoria ternatea leaves. The red fluorescent carbon dots (RCDs) were prepared by hydrothermal method, followed by lipid coating using rotary evaporation for imaging-guided phototherapy. RCDs generate heat in tandem with NIR laser irradiation and could therefore be employed as a photothermal agent in cancer therapy. Additionally, the fluorescent nature of RCDs can be utilized in bioimaging. The fabricated RCDs displayed a characteristic fluorescent emission maximum at 672 nm with a shoulder peak at 723 nm. Hydrophobicity is a major drawback associated with the RCDs, which limits their therapeutic efficiency due to poor biodistribution and rapid clearance. To address this limitation, we coated RCDs with soya lecithin to generate hydrophilic LRCDs with better bioavailability and therapeutic effectiveness. Further analysis using MTT assay reveals high biocompatibility and a distinct photothermal ablation potency of LRCDs against L929 and 4T1 cells, respectively. LRCDs could potentially be synthesized on a large scale and used for a variety of applications due to their low-cost, and biocompatibility.
利用植物叶片合成碳点是一种简便且经济可行的方法。在此，我们报告了一种脂质包覆的红色荧光碳点（LRCDs）的研发情况，它是一种生物相容性良好且稳定的纳米材料，其制备利用了蝶豆叶片。红色荧光碳点（RCDs）通过水热法制备，随后使用旋转蒸发进行脂质包覆，用于成像引导光疗。RCDs在近红外激光照射下会产生热量，因此可作为光热剂用于癌症治疗。此外，RCDs的荧光特性可用于生物成像。所制备的RCDs在672nm处显示出特征性荧光发射最大值，在723nm处有一个肩峰。疏水性是RCDs的一个主要缺点，由于其生物分布不佳和快速清除，限制了它们的治疗效率。为了解决这一限制，我们用大豆卵磷脂包覆RCDs，以生成具有更好生物利用度和治疗效果的亲水性LRCDs。使用MTT法进行的进一步分析表明，LRCDs分别对L929和4T1细胞具有高生物相容性和显著的光热消融能力。由于其低成本和生物相容性，LRCDs有可能大规模合成并用于多种应用。
