Department of Food Engineering, Sichuan University, Chengdu 610065, PR China.
Department of Food Engineering, Sichuan University, Chengdu 610065, PR China; The Key Laboratory of Food Science and Technology of Sichuan Province of Education, Sichuan University, Chengdu 610065, PR China.
Int J Biol Macromol. 2023 Dec 31;253(Pt 2):126792. doi: 10.1016/j.ijbiomac.2023.126792. Epub 2023 Sep 7.
A polysaccharide (EOP) from Epiphyllum oxypetalum (DC.) Haw. was isolated and identified, and its immunomodulatory activity was evaluated both in vitro and in vivo. By using multispectral analysis, EOP was determined to be composed of rhamnose, arabinose, galactose, and galacturonic acid at a molar ratio of 26.65:11.48:53.79:6.04, and its molecular weight was 5.77 × 10 Da. In addition, backbone structure of EOP was determined to consist of (1 → 4)-linked β-Galp, (1 → 2)-linked β-Rhap, (1 → 3,4)-linked β-Galp, (1 → 2,4)-linked β-Rhap and (1 → 4) -linked α-GalpA, terminating with t-β-Arap and t-β-Galp. The in vitro immunomodulatory activity assay on RAW 264.7 cell showed that EOP increased the proliferation of macrophages, enhanced its phagocytic capability, and promoted the production of cytokines including nitric oxide (NO), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6). Furthermore, the in vivo evaluation on zebrafish showed that EOP could reduce the residual content of fluorescent microspheres in zebrafish, which indicated that EOP had the capability to enhance the macrophage phagocytosis. All results suggested that EOP showed a complex structure and exhibited significant immunomodulatory activity both in vitro and in vivo that had the potential to be utilized valuably in food and medicine industries.
从 Epiphyllum oxypetalum (DC.) Haw. 中分离得到一种多糖（EOP），并对其进行了鉴定，同时评估了其在体内和体外的免疫调节活性。通过多光谱分析，确定 EOP 由鼠李糖、阿拉伯糖、半乳糖和半乳糖醛酸组成，摩尔比为 26.65:11.48:53.79:6.04，分子量为 5.77×10 Da。此外，EOP 的骨架结构被确定为由 (1→4)-β-Galp、(1→2)-β-Rhap、(1→3,4)-β-Galp、(1→2,4)-β-Rhap 和 (1→4)-α-GalpA 组成，以 t-β-Arap 和 t-β-Galp 结尾。在 RAW 264.7 细胞中的体外免疫调节活性测定表明，EOP 能增加巨噬细胞的增殖，增强其吞噬能力，并促进包括一氧化氮（NO）、肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）在内的细胞因子的产生。此外，在斑马鱼体内的评估表明，EOP 可以减少斑马鱼中荧光微球的残留含量，这表明 EOP 具有增强巨噬细胞吞噬作用的能力。所有结果表明，EOP 具有复杂的结构，并表现出显著的体内外免疫调节活性，具有在食品和医药行业中得到有价值应用的潜力。
