利用微生物混合物进行玉米赤霉烯酮的体外隔离。
Sequestration of zearalenone using microorganisms blend in vitro.
机构信息
Molecular Food Microbiology Laboratory, Department of Food Science, Purdue University, West Lafayette, IN 47907, USA.
Research and Development, United Animal Health, Sheridan, IN 46069, USA.
出版信息
Lett Appl Microbiol. 2023 Feb 16;76(2). doi: 10.1093/lambio/ovad020.
Zearalenone (ZEN) is an estrogenic mycotoxin produced by the Fusarium species and induces severe reproductive disorders in animals thus a major concern in the livestock industry. Probiotic bacteria treatments have been shown to inactivate mycotoxins, therefore, in this study, we investigated the effect of two commercial probiotic feed additives on the sequestration of ZEN. Commercial probiotic blends containing clay-based binder with Aspergillus niger, Bacillus licheniformis, Bacillus pumilus, and Bacillus subtilis at various proportions from BioMatrix International were incubated with ZEN in a time-dependent manner and then analyzed by Enzyme-Linked Immunosorbent Assay (ELISA) to quantify unbound ZEN. Sequestration of ZEN was further verified by using MCF-7 cell-based cytotoxicity and/or cell proliferation assays. ZEN, or probiotic mix, was nontoxic to MCF-7 cells. Probiotic blends decreased ZEN concentration by 45% (∼100 μg L-1) and prevented ZEN from inducing MCF-7 cell proliferation (20%-28% reduction). The probiotic feed supplements tested show a potential utility in ZEN neutralization.
玉米赤霉烯酮（ZEN）是一种由镰刀菌属产生的雌激素真菌毒素，会导致动物严重的生殖障碍，因此是畜牧业的主要关注点。益生菌细菌处理已被证明可以使真菌毒素失活，因此，在这项研究中，我们研究了两种商业益生菌饲料添加剂对 ZEN 螯合的影响。商业益生菌混合物含有基于粘土的粘合剂，以及黑曲霉、地衣芽孢杆菌、短小芽孢杆菌和枯草芽孢杆菌，其比例来自 BioMatrix International，与 ZEN 以时间依赖性方式孵育，然后通过酶联免疫吸附测定（ELISA）进行分析，以定量未结合的 ZEN。通过 MCF-7 基于细胞的细胞毒性和/或细胞增殖测定进一步验证了 ZEN 的螯合作用。ZEN 或益生菌混合物对 MCF-7 细胞没有毒性。益生菌混合物使 ZEN 浓度降低了 45%（约 100μg/L-1），并阻止 ZEN 诱导 MCF-7 细胞增殖（减少 20%-28%）。测试的益生菌饲料补充剂显示出在 ZEN 中和方面的潜在用途。
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