Pepoyan A Z, Manvelyan A M, Balayan M H, Harutyunyan N A, Tsaturyan V V, Batikyan H, Bren A B, Chistyakov V, Weeks R, Chikindas M L
Division of Food Safety and Biotechnology, Armenian National Agrarian University, 74 Teryan St, Yerevan 0009, Armenia.
The International Scientific-Educational Center of the National Academy of Sciences of the Republic of Armenia, 24d Marshal Baghramyan Ave, Yerevan 0019, Armenia.
Lett Appl Microbiol. 2023 Feb 16;76(2). doi: 10.1093/lambio/ovad021.
The ecological state of Lake Sevan, the largest drinking water reservoir for the South Caucasus, formed under the influence of climatic and social changes. This study assesses the bacteriological quality of water in the rivers of the Lake Sevan basin and tetracycline-resistant bacteria isolated from fish and people living near the rivers of the Lake Sevan basin in Armenia in autumn 2019 and spring 2020. No differences have been shown for the tetracycline resistance of the investigated E. coli isolated from the human gut and the Masrik, Argichi, and Gavaraget Rivers. Horizontal gel electrophoresis revealed the same plasmid bands in most of the investigated E. coli with the same tetracycline resistance from the different sources of the Argichi River (obtained from people/fish/water sources where the fish were caught). The results also showed that most of the waters carried Edwardsiella spp., Erwinia spp., Morganella spp., and Proteus spp. in addition to E. coli; the coliform index did not exceed the standard level of 5 × 104 CFU mL-1 there. These findings highlight the importance of multidisciplinary studies of bacteria from "interacting" ecosystems, which might serve as a basis for the suggestion of microbial antibiotic resistance as another indicator of water pollution.
塞凡湖是南高加索地区最大的饮用水水库，其生态状况是在气候和社会变化的影响下形成的。本研究评估了2019年秋季和2020年春季亚美尼亚塞凡湖流域河流中的水质细菌学质量，以及从塞凡湖流域河流附近的鱼类和居民中分离出的耐四环素细菌。从人类肠道以及马斯里克河、阿尔吉奇河和加瓦拉盖特河中分离出的受调查大肠杆菌的四环素抗性没有差异。水平凝胶电泳显示，从阿尔吉奇河不同来源（从人类/鱼类/捕鱼水域获取）分离出的具有相同四环素抗性的大多数受调查大肠杆菌中，质粒条带相同。结果还表明，除大肠杆菌外，大多数水体还携带爱德华氏菌属、欧文氏菌属、摩根氏菌属和变形杆菌属；那里的大肠菌群指数未超过5×104 CFU mL-1的标准水平。这些发现凸显了对来自“相互作用”生态系统的细菌进行多学科研究的重要性，这可能为将微生物抗生素抗性作为水污染的另一个指标提供建议依据。
