Institute of Biology, Leiden University, Leiden, Netherlands.
Front Immunol. 2023 Jan 18;13:1075473. doi: 10.3389/fimmu.2022.1075473. eCollection 2022.
is the most common nontuberculous mycobacterium (NTM) species causing infectious disease. Here, we characterized a infection model in zebrafish larvae, and compared it to infection, a model of tuberculosis. bacteria are efficiently phagocytosed and frequently induce granuloma-like structures in zebrafish larvae. Although macrophages can respond to both mycobacterial infections, their migration speed is faster in infections caused by . Tlr2 is conservatively involved in most aspects of the defense against both mycobacterial infections. However, Tlr2 has a function in the migration speed of macrophages and neutrophils to infection sites with that is not observed with . Using RNAseq analysis, we found a distinct transcriptome response in cytokine-cytokine receptor interaction for and infection. In addition, we found differences in gene expression in metabolic pathways, phagosome formation, matrix remodeling, and apoptosis in response to these mycobacterial infections. In conclusion, we characterized a new infection model in zebrafish that can be further used in studying pathological mechanisms for NTM-caused diseases.
是引起感染性疾病的最常见非结核分枝杆菌（NTM）物种。在这里，我们建立了一个 感染斑马鱼幼虫的模型，并将其与 感染（结核病模型）进行了比较。 细菌被有效地吞噬，并经常在斑马鱼幼虫中诱导肉芽肿样结构。虽然巨噬细胞可以对两种分枝杆菌感染做出反应，但 在 感染中，它们向感染部位的迁移速度更快。Tlr2 在抵抗两种分枝杆菌感染的大多数方面都保守参与。然而，Tlr2 在 引起的巨噬细胞和中性粒细胞向感染部位迁移的速度方面具有功能，而在 感染中则没有观察到这种功能。通过 RNAseq 分析，我们发现细胞因子-细胞因子受体相互作用在 和 感染中存在明显的转录组反应。此外，我们发现这些分枝杆菌感染在代谢途径、吞噬体形成、基质重塑和细胞凋亡方面的基因表达存在差异。总之，我们建立了一个新的 感染斑马鱼的模型，可进一步用于研究 NTM 引起的疾病的病理机制。
