Kengne Irene Chinda, Fankam Aimé Gabriel, Yamako Elodie Konack, Tamokou Jean-De-Dieu
Research Unit of Microbiology and Antimicrobial Substances, Faculty of Sciences, University of Dschang, Dschang, P.O. Box 67, Cameroon.
Département des Sciences Appliquées à la Santé, Institut Universitaire et Stratégique de l'Estuaire (IUEs/Insam), BP 4100, Douala, Cameroon.
Adv Pharmacol Pharm Sci. 2023 Jan 25;2023:2565857. doi: 10.1155/2023/2565857. eCollection 2023.
Phytochemicals present in medicinal plants (herbs, shrubs, and trees) are endowed with high antimicrobial and antioxidant properties. The aim of this work was to study the chemical composition, antioxidant, and antifungal activities of , and Chemical composition of the plant extracts was determined using standard methods. The antioxidant activities were performed using 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), nitric oxide (NO), and hydroxyl (OH) scavenging assays. The antifungal activity of plant extracts and their combinations with antifungals was evaluated against eleven . using the broth microdilution method by determining the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC). The quantitative chemical analysis of the extracts of , and showed that they contain phenols, tannins, and flavonoids that vary according to the plant species and extracts. All the plant extracts presented promising antifungal (MIC = 64-2048 g/mL) and antioxidant activities. The extract of displayed the highest antifungal (MIC = 64-256 g/mL) and antioxidant (IC = 19.052 ± 1.11 g/mL) activities which can be explained by its high phenolic content. Interestingly, extracts of , , and displayed synergistic effects (fractional inhibitory concentration index, FICI ≤ 0.5) with ketoconazole against clinical resistant isolates. The results of the present study demonstrate promising antifungal and antioxidant activities of the tested plants that are associated to their phenol, tannin, and flavonoid contents. Hence, extracts of and could be deeply investigated as antifungal alone and in combination with conventional antifungal drugs to treat infections caused by .
药用植物（草本、灌木和树木）中含有的植物化学物质具有很高的抗菌和抗氧化特性。本研究的目的是研究[植物名称1]、[植物名称2]和[植物名称3]的化学成分、抗氧化和抗真菌活性。采用标准方法测定植物提取物的化学成分。通过2,2-二苯基-1-苦基肼（DPPH）、铁还原抗氧化能力（FRAP）、一氧化氮（NO）和羟基（OH）清除试验来测定抗氧化活性。采用肉汤微量稀释法，通过测定最低抑菌浓度（MIC）和最低杀菌浓度（MFC），评估植物提取物及其与抗真菌剂组合对11种[真菌名称]的抗真菌活性。对[植物名称1]、[植物名称2]和[植物名称3]提取物的定量化学分析表明，它们含有酚类、单宁和黄酮类化合物，其含量因植物种类和提取物而异。所有植物提取物均表现出良好的抗真菌（MIC = 64 - 2048μg/mL）和抗氧化活性。[植物名称1]的提取物表现出最高的抗真菌（MIC = 64 - 256μg/mL）和抗氧化（IC50 = 19.052±1.11μg/mL）活性，这可以通过其高酚含量来解释。有趣的是，[植物名称2]、[植物名称3]和[植物名称4]的提取物与酮康唑对临床耐药菌株表现出协同作用（部分抑菌浓度指数，FICI≤0.5）。本研究结果表明，受试植物具有良好的抗真菌和抗氧化活性，这与它们的酚类、单宁和黄酮类化合物含量有关。因此，[植物名称1]和[植物名称2]的提取物可作为单一抗真菌剂以及与传统抗真菌药物联合用于治疗由[真菌名称]引起的感染进行深入研究。
