Deng Bin, Gu Xiaoyan, Chen Sen, Zhang Meng, Hao Suwei, Wei Lixian, Cao Ying, Hu Shanglian
Lab of Plant Cell Engineering, Southwest University of Science and Technology, Mianyang, Sichuan, China.
Engineering Research Center for Biomass Resource Utilizaiton and Modification of Sichuan Province, Mianyang, Sichuan, China.
Front Plant Sci. 2023 Jan 20;13:1118398. doi: 10.3389/fpls.2022.1118398. eCollection 2022.
Sucrose is the main transported form of photosynthetic products. Sucrose transporter (SUT) participates in the translocation of sucrose from source to sink, which is important for the growth and development of plants. is an important economic crop in southwestern China because of its high growth rate, high fiber content, and dual usage for food and timber, but the mechanism of sucrose transportation in is unclear. In this study, a total of 12 transporter genes were determined in by whole-genome identification. , , and were homologs of rice , while was a homolog of , and these four genes were expressed in the leaf, internode, node, and bamboo shoots of . In addition, family genes were involved in photosynthetic product distribution, ABA/MeJA responses, and drought resistance, especially . The function of was then verified in . DfSUT4 was localized mainly in the leaf mesophyll and stem phloem of transgenic plant. The overexpression of gene in transgenic plant showed increases of photosynthetic rate, above-ground biomass, thousand grain weight, and cellulose content. Our findings altogether indicate that can be a candidate gene that can be involved in phloem sucrose transportation from the source leaves to the sink organs, phytohormone responses, abiotic stress, and fiber formation in plants, which is very important in the genetic improvement of and other crops.
蔗糖是光合产物的主要运输形式。蔗糖转运蛋白（SUT）参与蔗糖从源到库的转运，这对植物的生长发育至关重要。由于其生长速度快、纤维含量高以及兼具食用和木材两用性，[作物名称未明确]是中国西南部一种重要的经济作物，但[作物名称未明确]中蔗糖运输的机制尚不清楚。在本研究中，通过全基因组鉴定在[作物名称未明确]中总共确定了12个蔗糖转运蛋白基因。[基因名称未明确]、[基因名称未明确]和[基因名称未明确]是水稻[基因名称未明确]的同源物，而[基因名称未明确]是[基因名称未明确]的同源物，这四个蔗糖转运蛋白基因在[作物名称未明确]的叶片、节间、节和竹笋中表达。此外，蔗糖转运蛋白家族基因参与光合产物分配、脱落酸/茉莉酸甲酯反应和抗旱性，尤其是[基因名称未明确]。然后在[作物名称未明确]中验证了[基因名称未明确]的功能。DfSUT4主要定位于[作物名称未明确]转基因植物的叶片叶肉和茎韧皮部。[基因名称未明确]基因在转基因植物中的过表达显示光合速率、地上生物量、千粒重和纤维素含量增加。我们的研究结果共同表明，[基因名称未明确]可以作为一个候选基因，参与植物中从源叶到库器官的韧皮部蔗糖运输、植物激素反应、非生物胁迫和纤维形成，这在[作物名称未明确]和其他作物的遗传改良中非常重要。
