Firat Huseyin, Zhang Lu, Baksi Shounak, Leszek Przemyslaw, Schordan Eric, Ounzain Samir, Kottwitz Jan, Patriki Dimitri, Heidecker Bettina, Lüscher Thomas F, Pedrazzini Thierry, Devaux Yvan
Firalis SA, Huningue, France.
Cardiovascular Research Unit, Luxembourg Institute of Health, Strassen, Luxembourg.
Heliyon. 2023 Jan 20;9(1):e13087. doi: 10.1016/j.heliyon.2023.e13087. eCollection 2023 Jan.
Cardiovascular disorders such as heart failure are leading causes of mortality. Patient stratification via identification of novel biomarkers could improve management of cardiovascular diseases of complex etiologies. Long-noncoding RNAs (lncRNAs) are highly tissue-specific in nature and have emerged as important biomarkers in human diseases. In this study, we aimed to identify cardiac-enriched lncRNAs as potential biomarkers for cardiovascular conditions. Deep RNA sequencing and quantitative PCR identified differentially expressed lncRNAs between failing and non-failing hearts. An independent dataset was used to evaluate the enrichment of lncRNAs in normal hearts. We identified a panel of 2906 lncRNAs, named FIMICS, that were either cardiac-enriched or differentially expressed between failing and non-failing hearts. Expression of lncRNAs in blood samples differentiated patients with myocarditis and acute myocardial infarction. We hereby present the FIMICS panel, a readily available tool to provide insights into cardiovascular pathologies and which could be helpful for diagnosis, monitoring and prognosis purposes.
心力衰竭等心血管疾病是主要的死亡原因。通过识别新型生物标志物进行患者分层，可改善复杂病因心血管疾病的管理。长链非编码RNA（lncRNA）本质上具有高度的组织特异性，并已成为人类疾病中的重要生物标志物。在本研究中，我们旨在鉴定心脏富集的lncRNA作为心血管疾病的潜在生物标志物。深度RNA测序和定量PCR确定了衰竭心脏和非衰竭心脏之间差异表达的lncRNA。使用一个独立数据集评估lncRNA在正常心脏中的富集情况。我们鉴定出一组2906个lncRNA，命名为FIMICS，它们要么是心脏富集的，要么在衰竭心脏和非衰竭心脏之间差异表达。lncRNA在血液样本中的表达可区分心肌炎和急性心肌梗死患者。我们在此展示FIMICS组，这是一个现成的工具，可深入了解心血管病理情况，有助于诊断、监测和预后。
