Han Siyin, Huang Delun, Lan Taijin, Wu Yongpei, Wang Yingbiao, Wei Jiying, Zhang Weiyuan, Ou Yuanyang, Yan Qiangqiang, Liu Peng, Chen Zhenxing, Lin Jiang
School of Basic Medicine, Guangxi University of Chinese Medicine, Nanning, China.
Evid Based Complement Alternat Med. 2021 Dec 9;2021:9516427. doi: 10.1155/2021/9516427. eCollection 2021.
The objective of this study was to investigate the therapeutic effect of seawater pearl powder (SPP) on ultraviolet (UV) irradiation-induced photoaging in mouse skin. The protein and trace elements in SPP were detected by liquid chromatography-mass spectrometry, atomic fluorescence spectrometry, and inductively coupled plasma-atomic emission spectrometry. The effect of SPP on treating skin damage resulting from UV-induced photoaging was observed by gross physical appearance and histopathological analysis. Oxidative stress and melanin synthesis were analyzed using biochemical method. Western blotting was applied to analyze the phosphorylation and expression levels of matrix metalloproteinase-1 (MMP-1), collagen I, and proteins involved in the mitogen-activated protein kinase (MAPK) signaling pathways (p38, ERK, and JNK). The results show that SPP has a significant therapeutic effect on UV-induced photoaging of skin and improves and restores appearance and tissue structure of mouse skin. The major mechanism may be related to reduction of expression level of MMP-1 and enhancement of collagen I production via inhibition of MAPK signaling pathway after scavenging of excess reactive oxygen species (ROS) in the UV-induced photoaged skin of mice. Meanwhile, it may also be involved in reducing melanin content by inhibiting tyrosinase activity after scavenging excess ROS in the UV-induced photoaged skin of mice. Therefore, SPP could be a good substance to treat photoaging skin. Taking cost-effectiveness and efficacy into consideration, the optimal concentration of SPP for treating photoaging skin could be 100 mg/g.
本研究的目的是探讨海水珍珠粉（SPP）对紫外线（UV）照射诱导的小鼠皮肤光老化的治疗效果。通过液相色谱-质谱联用、原子荧光光谱法和电感耦合等离子体原子发射光谱法检测SPP中的蛋白质和微量元素。通过大体外观和组织病理学分析观察SPP对UV诱导的光老化所致皮肤损伤的治疗效果。采用生化方法分析氧化应激和黑色素合成情况。应用蛋白质印迹法分析基质金属蛋白酶-1（MMP-1）、I型胶原蛋白以及丝裂原活化蛋白激酶（MAPK）信号通路（p38、ERK和JNK）相关蛋白的磷酸化和表达水平。结果表明，SPP对UV诱导的皮肤光老化具有显著治疗作用，可改善和恢复小鼠皮肤的外观和组织结构。主要机制可能与清除小鼠UV诱导的光老化皮肤中过量的活性氧（ROS）后，通过抑制MAPK信号通路降低MMP-1表达水平并增强I型胶原蛋白生成有关。同时，在清除小鼠UV诱导的光老化皮肤中过量的ROS后，它还可能通过抑制酪氨酸酶活性参与降低黑色素含量。因此，SPP可能是治疗光老化皮肤的良好物质。综合考虑成本效益和疗效，治疗光老化皮肤的SPP最佳浓度可能为100 mg/g。
