Negrin S, Nefelova M V, Gavrilova E M, Morales S, Egorov N S
Antibiot Med Biotekhnol. 1987 Aug;32(8):579-83.
The effect of potassium orthophosphate on growth of the mycelium, its ATP contents and biosynthesis of the macrotetrolide antibiotic nonactin by Str. chrysomallus var. macrotetrolidi was studied. Direct dependence of the ATP contents in the mycelium on the amount of the phosphate added to the medium and consumed by the developing actinomycete was shown. Changes in the intracellular content of ATP depended also on the mycelium age. It was characterized by two peaks. Hemin was detected in the actinomycete mycelium. Its levels were sufficiently high and depended on the mycelium age and cultivation conditions, in particular on the phosphate content in the medium. Higher levels of nonactin biosynthesis were characteristic of the mycelium with lower contents of ATP, proteins and hemin. Intensive production of the antibiotic proceeded at the background of decreasing levels of ATP in the mycelium.
研究了正磷酸钾对大孢黄绿链霉菌变种产生的大环状四醇类抗生素诺菌素的菌丝体生长、ATP含量及其生物合成的影响。结果表明，菌丝体中ATP含量与添加到培养基中并被生长中的放线菌消耗的磷酸盐量直接相关。ATP的细胞内含量变化也取决于菌丝体的年龄，其特点是有两个峰值。在放线菌菌丝体中检测到了血红素，其含量足够高，并且取决于菌丝体的年龄和培养条件，特别是培养基中的磷酸盐含量。ATP、蛋白质和血红素含量较低的菌丝体具有较高水平的诺菌素生物合成。抗生素的大量生产是在菌丝体中ATP水平下降的背景下进行的。
