[Macrotetrolide antibiotics from a recombinant strain of Streptococcus chrysomallus].

A mixture of antibiotic substances was isolated from the culture fluid of a recombinant strain of Streptomyces chrysomallus. The substances were identified as macrotetrolides (nonactin, monactin, dinactin and trinactin) by the mass spectrometry, ionoform properties and thin layer chromatography in the antibiotic samples and ethylacetate extracts of the fermentation broth filtrate. The parent strain of Streptomyces chrysomallus also synthesized a mixture of macrotetrolides. However, it contained no nonactin and the total level of the macrotetrolide biosynthesis was 5 to 7 time lower than that in the recombinant strain (determined by a chemical method).