Orlova T I
Antibiot Khimioter. 1993 Jul;38(7):3-6.
A mixture of antibiotic substances was isolated from the culture fluid of a recombinant strain of Streptomyces chrysomallus. The substances were identified as macrotetrolides (nonactin, monactin, dinactin and trinactin) by the mass spectrometry, ionoform properties and thin layer chromatography in the antibiotic samples and ethylacetate extracts of the fermentation broth filtrate. The parent strain of Streptomyces chrysomallus also synthesized a mixture of macrotetrolides. However, it contained no nonactin and the total level of the macrotetrolide biosynthesis was 5 to 7 time lower than that in the recombinant strain (determined by a chemical method).
从金色链霉菌重组菌株的培养液中分离出了一种抗生素物质混合物。通过对抗生素样品以及发酵液滤液的乙酸乙酯提取物进行质谱分析、离子形式特性分析和薄层色谱分析，确定这些物质为大环四醇类抗生素（缬氨霉素、单活菌素、双活菌素和三活菌素）。金色链霉菌的亲本菌株也能合成大环四醇类抗生素混合物。然而，该亲本菌株不含缬氨霉素，且大环四醇类抗生素生物合成的总量比重组菌株低5至7倍（通过化学方法测定）。
