Lv Xianglian, Li Yinmei, Cheng Yanpeng, Lai Xin-He, Yang Jing, Lu Shan, Zhang Gui, Yang Caixin, Jin Dong, Liu Liyun, Xu Jianguo
Department of Epidemiology, Shanxi Medical University School of Public Health, Taiyuan, Shanxi, PR China.
State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, PR China.
Int J Syst Evol Microbiol. 2022 Dec;72(12). doi: 10.1099/ijsem.0.005633.
Four Gram-stain-positive, facultatively anaerobic, non-motile, non-spore-forming and rod-shaped bacteria (lx-72, lx-45, ZJ784 and ZJ955) were isolated from the respiratory tract or faeces of marmot () from the Qinghai-Tibet Plateau in China. Analysis of the 16S rRNA gene sequences showed that all strains belong to the genus and are more related to CCUG 64069 (95.1-97.4 % similarity) than to the genus . Both strain pairs grew well at pH 6-9 and 15-42°C, and ZJ784/ZJ955 could tolerate slightly higher NaCl (0.5-4.5 %, w/v) than lx-72/lx-45(0.5-3.5 %). Based on whole-genome sequences, the average nucleotide identity and digital DNA-DNA hybridization values between our four isolates and their closest relative were below the species delineation thresholds of 70 % and 95-96 %. The common major fatty acids (>10 %) of our four strains were anteiso-C and anteiso-C. For both new type strains, MK-8(H) and MK-9(H) were the major isoprenoid quinones, and diphosphatidylglycerol and phosphatidylglycerol were the main polar lipids. The genomic DNA G+C content of all strains was 53.9 mol%. Based on results from the genomic comparison, phylogenetic analysis, and physiological and biochemical characteristics, the four isolates represent two novel species in the genus , for which the names sp. nov. (type strain lx-72=KCTC 49658=GDMCC 1.2569) and sp. nov. (type strain ZJ784=KCTC 49507=GDMCC 1.1997) are proposed.
从中国青藏高原旱獭的呼吸道或粪便中分离出4株革兰氏阳性、兼性厌氧、无运动性、不产芽孢的杆状细菌(lx-72、lx-45、ZJ784和ZJ955)。16S rRNA基因序列分析表明,所有菌株均属于该属,与CCUG 64069的亲缘关系比与该属的关系更为密切(相似性为95.1-97.4%)。两对菌株在pH 6-9和15-42°C条件下生长良好,ZJ784/ZJ955比lx-72/lx-45能耐受略高浓度的NaCl(0.5-4.5%,w/v)(0.5-3.5%)。基于全基因组序列,我们的4株分离株与其最接近的亲缘种之间的平均核苷酸同一性和数字DNA-DNA杂交值低于70%和95-96%的物种界定阈值。我们4株菌株的常见主要脂肪酸(>10%)为anteiso-C和anteiso-C。对于两种新型菌株,MK-8(H)和MK-9(H)是主要的类异戊二烯醌,二磷脂酰甘油和磷脂酰甘油是主要的极性脂质。所有菌株的基因组DNA G+C含量为53.9mol%。基于基因组比较、系统发育分析以及生理生化特征的结果,这4株分离株代表了该属中的两个新物种,分别命名为sp. nov.(模式菌株lx-72=KCTC 49658=GDMCC 1.2569)和sp. nov.(模式菌株ZJ784=KCTC 49507=GDMCC 1.1997)。