State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, PR China.
Shanghai Institute for Emerging and Re-emerging Infectious Diseases, Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, PR China.
Int J Syst Evol Microbiol. 2022 Oct;72(10). doi: 10.1099/ijsem.0.005533.
Six Gram-stain-positive, aerobic or facultative anaerobic, catalase-positive, urease- and oxidase-negative, rod-shaped bacteria (zg-ZUI157/zg-ZUI40, zg-ZUI222/zg-ZUI199 and zg-ZUI188/ zg-ZUI168) were characterized by a polyphasic approach. Optimal growth of the six strains was observed at pH 7.0 and 28 °C. Phylogenetic analyses based on the 16S rRNA gene and 247 core genes revealed that they belong to genus . The three type strains have low digital DNA-DNA hybridization (19.3-30.1%) and average nucleotide identity values (78.0-85.5%) with all available genomes in the genus , and a DNA G+C content range of 73.0-74.6 mol%. The major fatty acids detected in strain pairs zg-ZUI157/zg-ZUI40 and zg-ZUI 222/zg-ZUI199 were C, anteiso-C and anteiso A-C, and C, anteiso-C, anteiso A-C and anteiso-C in strain pair zg-ZUI188/zg-ZUI168. Diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol mannosides were the major polar lipids detected in the three novel species. MK-9(H) was the predominant quinone detected in strains zg-ZUI222 (87.4 %) and zg-ZUI188 (91.4 %), and MK-9(H) (49.1 %) and MK-8 (43.4 %) in strain zg-ZUI157. The cell-wall sugars detected in the three novel species mainly contained rhamnose. The cell-wall peptidoglycan type of the three novel species was A4, with an inferred l-Orn-d-Asp interpeptide bridge for strains zg-ZUI157 and zg-ZUI222, and l-Orn-d-Glu for strain zg-ZUI188. Based on the results of the phenotypic, phylogenetic, genomic hybridization, average nucleotide identity and chemotaxonomic analyses, the six strains should be classified as belonging to three novel species, for which the names sp. nov. (zg-ZUI157=GDMCC 1.2559=KCTC 49678), sp. nov. (zg-ZUI222=GDMCC 1.2501=KCTC 49675) and sp. nov. (zg-ZUI188=GDMCC 1.2563=KCTC 49674) are proposed.
六株革兰氏阳性、需氧或兼性厌氧、过氧化氢酶阳性、脲酶和氧化酶阴性、杆状的细菌（zg-ZUI157/zg-ZUI40、zg-ZUI222/zg-ZUI199 和 zg-ZUI188/zg-ZUI168）通过多相分类法进行了表征。六株菌的最佳生长条件为 pH7.0 和 28°C。基于 16S rRNA 基因和 247 个核心基因的系统发育分析表明，它们属于 属。三个模式菌株与属内所有可获得的基因组的数字 DNA-DNA 杂交率（19.3-30.1%）和平均核苷酸同一性值（78.0-85.5%）较低，且 DNA G+C 含量范围为 73.0-74.6mol%。在菌株对 zg-ZUI157/zg-ZUI40 和 zg-ZUI 222/zg-ZUI199 中检测到的主要脂肪酸为 C、anteiso-C 和 anteiso A-C，而在菌株对 zg-ZUI188/zg-ZUI168 中检测到的主要脂肪酸为 C、anteiso-C、anteiso A-C 和 anteiso-C。在这三个新物种中，检测到的主要极性脂为双磷脂酰甘油、磷脂酰甘油和磷脂酰肌醇甘露糖苷。在菌株 zg-ZUI222（87.4%）和 zg-ZUI188（91.4%）中主要醌为 MK-9（H），在菌株 zg-ZUI157 中主要醌为 MK-9（H）（49.1%）和 MK-8（43.4%）。在三个新物种中检测到的细胞壁糖主要含有鼠李糖。三个新物种的细胞壁肽聚糖类型均为 A4，菌株 zg-ZUI157 和 zg-ZUI222 的推测为 l-Orn-d-Asp 间肽桥，而菌株 zg-ZUI188 的为 l-Orn-d-Glu。基于表型、系统发育、基因组杂交、平均核苷酸同一性和化学生态分析的结果，这 6 株菌应归类为三个新的 种，分别命名为. sp. nov.（zg-ZUI157=GDMCC 1.2559=KCTC 49678）、. sp. nov.（zg-ZUI222=GDMCC 1.2501=KCTC 49675）和. sp. nov.（zg-ZUI188=GDMCC 1.2563=KCTC 49674）。
