sp. nov. and sp. nov., new members in the genus .

Four novel bacterial strains (zg-ZUI122/zg-ZUI10 and zg-ZUI227/zg-ZUI100) were isolated from the intestinal contents of and characterized using a polyphasic approach. Cells were Gram-stain- and catalase-positive, urease- and oxidase-negative. Strains grew optimally at 28-30 °C, pH 7.0, with 0.5 % NaCl (w/v). A comparative analysis of 16S rRNA gene sequences revealed that strain pairs zg-ZUI122/zg-ZUI10 and zg-ZUI227/zg-ZUI100 belonged to the genus and were most closely related to DSM 20133, with similarities of 99.6 and 99.5 %, respectively. This was further confirmed by phylogenetic analyses based on the 16S rRNA gene and genome sequences. The digital DNA-DNA hybridization and average nucleotide identity values between the two new type strains (zg-ZUI122 and zg-ZUI227) and other species in the genus were 20.0-24.4/77.2-83.4% and 19.9-25.1/77.1-83.4%, all below the thresholds. The major cellular fatty acids detected in the two novel species included -C and -C; the predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. MK-8(H) (77.3%) was the predominant respiratory quinone detected in strain zg-ZUI122, while MK-8(H) (53.7%) and MK-9(H) (46.3%) were detected in strain zg-ZUI227. The shared cell-wall amino acids detected in the two novel species were alanine, glutamic acid and lysine; the shared whole cell wall sugars consisted of galactose, mannose and ribose. All these analyses concluded that these four strains represent two different novel species in the genus , for which the names sp. nov. (zg-ZUI122 = GDMCC 1.2502 = KCTC 49677) and sp. nov. (zg-ZUI227 = GDMCC 1.2500 = KCTC 49676) are proposed.