State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, PR China.
Shanghai Institute for Emerging and Re-emerging Infectious Diseases, Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, PR China.
Int J Syst Evol Microbiol. 2022 Feb;72(2). doi: 10.1099/ijsem.0.005181.
Four novel bacterial strains (zg-ZUI122/zg-ZUI10 and zg-ZUI227/zg-ZUI100) were isolated from the intestinal contents of and characterized using a polyphasic approach. Cells were Gram-stain- and catalase-positive, urease- and oxidase-negative. Strains grew optimally at 28-30 °C, pH 7.0, with 0.5 % NaCl (w/v). A comparative analysis of 16S rRNA gene sequences revealed that strain pairs zg-ZUI122/zg-ZUI10 and zg-ZUI227/zg-ZUI100 belonged to the genus and were most closely related to DSM 20133, with similarities of 99.6 and 99.5 %, respectively. This was further confirmed by phylogenetic analyses based on the 16S rRNA gene and genome sequences. The digital DNA-DNA hybridization and average nucleotide identity values between the two new type strains (zg-ZUI122 and zg-ZUI227) and other species in the genus were 20.0-24.4/77.2-83.4% and 19.9-25.1/77.1-83.4%, all below the thresholds. The major cellular fatty acids detected in the two novel species included -C and -C; the predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. MK-8(H) (77.3%) was the predominant respiratory quinone detected in strain zg-ZUI122, while MK-8(H) (53.7%) and MK-9(H) (46.3%) were detected in strain zg-ZUI227. The shared cell-wall amino acids detected in the two novel species were alanine, glutamic acid and lysine; the shared whole cell wall sugars consisted of galactose, mannose and ribose. All these analyses concluded that these four strains represent two different novel species in the genus , for which the names sp. nov. (zg-ZUI122 = GDMCC 1.2502 = KCTC 49677) and sp. nov. (zg-ZUI227 = GDMCC 1.2500 = KCTC 49676) are proposed.
从 的肠道内容物中分离到 4 株新型细菌（zg-ZUI122/zg-ZUI10 和 zg-ZUI227/zg-ZUI100），并采用多相方法进行了表征。细胞革兰氏染色阳性，过氧化氢酶阳性，脲酶和氧化酶阴性。菌株在 28-30°C、pH7.0 和 0.5%NaCl（w/v）条件下生长最佳。16S rRNA 基因序列的比较分析表明，菌株对 zg-ZUI122/zg-ZUI10 和 zg-ZUI227/zg-ZUI100 属于 属，与 DSM 20133 的相似度最高，分别为 99.6%和 99.5%。这进一步通过基于 16S rRNA 基因和基因组序列的系统发育分析得到证实。两个新菌株（zg-ZUI122 和 zg-ZUI227）与属内其他种之间的数字 DNA-DNA 杂交和平均核苷酸同一性值分别为 20.0-24.4/77.2-83.4%和 19.9-25.1/77.1-83.4%，均低于阈值。在这两个新种中检测到的主要细胞脂肪酸包括 -C 和 -C；主要的极性脂是双磷脂酰甘油、磷脂酰甘油和磷脂酰肌醇。在 zg-ZUI122 菌株中检测到主要的呼吸醌为 MK-8(H)（77.3%），而在 zg-ZUI227 菌株中检测到 MK-8(H)（53.7%）和 MK-9(H)（46.3%）。在这两个新种中检测到的共享细胞壁氨基酸为丙氨酸、谷氨酸和赖氨酸；共享的整个细胞壁糖由半乳糖、甘露糖和核糖组成。所有这些分析表明，这 4 株菌代表 属中的两个不同的新种，分别命名为 sp. nov.（zg-ZUI122=GDMCC 1.2502=KCTC 49677）和 sp. nov.（zg-ZUI227=GDMCC 1.2500=KCTC 49676）。
