Chen Yongqing, Liu Tianqi, Lai Qiliang, Dong Chunming, Shao Zongze
Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources of China; State Key Laboratory Breeding Base of Marine Genetic Resources; Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen 361005, PR China.
College of Biological Science and Engineering, Fuzhou University, Fuzhou 350108, PR China.
Int J Syst Evol Microbiol. 2022 Nov;72(11). doi: 10.1099/ijsem.0.005606.
is a group of marine bacteria with important industrial application potential and ecological functions. In this study, a Gram-stain-negative, rod-shaped, non-motile, strictly aerobic and bright yellow pigmented bacterial strain within this genus, designated C2-37M9, was isolated from a surface seawater sample from the Philippine Basin in the Western Pacific Ocean. Strain C2-37M9 grew at 10-44 °C (optimum, 28-30 °C), pH 6-9 (pH 7) and in the presence of 0-12 % NaCl (w/v; 2-3 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that it belonged to the genus and had 95.7-98.7 % sequence similarity to all type strains of this genus, with the highest value corresponding to (98.7 %). Digital DNA-DNA hybridization, average nucleotide identity and average amino acid identity values between strain C2-37M9 and all valid type strains were 27.5-32.3, 83.8-86.7 and 86.9-89.0 %, respectively. The principal fatty acids (>5 %) were iso-C, iso-C 3-OH, anteiso-C, summed feature 9 (C 10-methyl and/or iso-C 9), iso-C G and summed feature 3 (C 6 and/or C 7). The predominant respiratory quinone was MK-6. The polar lipids were one phosphatidylethanolamine, two unknown glycolipids, three unidentified aminolipids and six unidentified lipids. The genomic DNA G+C content of strain C2-37M9 was 36.7 mol%. Based on phylogenetic results and genomic-based relatedness indices, as well as phenotypic and genotypic characteristics, strain C2-37M9 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is C2-37M9 (=MCCC M21534=KCTC 82852).
是一类具有重要工业应用潜力和生态功能的海洋细菌。在本研究中，从西太平洋菲律宾海盆的表层海水样本中分离出该属内一株革兰氏染色阴性、杆状、无运动性、严格需氧且呈亮黄色色素的菌株，命名为C2 - 37M9。菌株C2 - 37M9在10 - 44 °C（最适温度为28 - 30 °C）、pH 6 - 9（pH 7）以及0 - 12 % NaCl（w/v；2 - 3 %）存在的条件下生长。基于16S rRNA基因序列的系统发育分析表明，它属于该属，与该属所有模式菌株的序列相似性为95.7 - 98.7 %，最高值对应于（98.7 %）。菌株C2 - 37M9与所有有效模式菌株之间的数字DNA - DNA杂交、平均核苷酸同一性和平均氨基酸同一性值分别为27.5 - 32.3、83.8 - 86.7 和86.9 - 89.0 %。主要脂肪酸（>5 %）为异-C、异-C 3-OH、anteiso-C、总和特征9（C 10-甲基和/或异-C 9）、异-C G和总和特征3（C 6和/或C 7）。主要呼吸醌为MK - 6。极性脂为一种磷脂酰乙醇胺、两种未知糖脂、三种未鉴定氨基脂和六种未鉴定脂。菌株C2 - 37M9的基因组DNA G + C含量为36.7 mol%。基于系统发育结果、基于基因组的相关性指数以及表型和基因型特征，菌株C2 - 37M9代表该属内的一个新物种，为此提出新物种名为 sp. nov.。模式菌株为C2 - 37M9（=MCCC M21534=KCTC 82852）。
