ABS Research Support Center, KRIBB, 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea.
Vitabio, Inc., Daejeon, 305-500, Republic of Korea.
Anaerobe. 2023 Dec;84:102792. doi: 10.1016/j.anaerobe.2023.102792. Epub 2023 Nov 2.
The family Lachnospiraceae is affiliated with the order Clostridiales and was originally contained within Clostridial cluster XIVa. The members of Lachnospiraceae inhabiting the gut comprise the chemoorganotrophic genera, generating sundry short-chain fatty acids to supply energy to the host, and are considered to be related to obesity and gut health.
The polyphasic taxonomic approach was used to characterize the isolate YH-rum2234. A detailed metabolic analysis was conducted to compare the novel isolate with related strains within the family Lachnospiraceae.
A fusiform, obligately anaerobic, Gram-stain-negative bacterium, YH-rum2234, was isolated from pig feces. Analysis of the 16S rRNA gene sequence revealed that the similarities between the isolate and the familiarly interrelated strain Lientehia hominis KCTC 25345 was 94.3%. The average nucleotide identities and genome-to-genome distances of YH-rum2234 and its closely related strains were below 85.5% and 32.5%, respectively. The G + C content of the genomic DNA was 49.2 mol%. The main fatty acids were C, C, and C DMA. The major polar lipids were aminophospholipids. The cell wall did not contain the peptidoglycan meso-diaminopimelic acid.
Given the chemotaxonomic, phenotypic, and phylogenetic properties, YH-rum2234 (=KCTC 25710 = DSMZ 116041) represents a new genus and species in the family Lachnospiraceae. Fusibacillus kribbianus gen. nov., sp. nov. is the proposed name.
厚壁菌门的毛螺菌科最初被归入梭菌目梭菌 XIVa 簇，该科的成员栖息在肠道中，包括化能有机营养菌属，产生各种短链脂肪酸为宿主提供能量，被认为与肥胖和肠道健康有关。
采用多相分类学方法对分离株 YH-rum2234 进行了表征。对该新分离株进行了详细的代谢分析，并与毛螺菌科内的相关菌株进行了比较。
从猪粪便中分离到一株梭形、严格厌氧、革兰氏阴性细菌 YH-rum2234。16S rRNA 基因序列分析表明，该分离株与亲缘关系密切的菌株 Lientehia hominis KCTC 25345 的相似度为 94.3%。YH-rum2234 及其密切相关菌株的平均核苷酸同一性和全基因组距离分别低于 85.5%和 32.5%。基因组 DNA 的 G+C 含量为 49.2mol%。主要脂肪酸为 C、C 和 C DMA。主要极性脂为氨基磷脂。细胞壁不含肽聚糖 meso-二氨基庚二酸。
根据化学分类学、表型和系统发育特性，YH-rum2234（=KCTC 25710=DSMZ 116041）代表了毛螺菌科中的一个新属和新种。 Fusibacillus kribbianus gen. nov.，sp. nov. 是建议的名称。
