Laboratory of Marine Biodiversity Research, Third Institute of Oceanography, Ministry of Natural Resources, 178 Daxue Road, Xiamen 361005, PR China.
College of Marine Life Sciences, Ocean University of China, Qingdao 266003, PR China.
Int J Syst Evol Microbiol. 2023 Nov;73(11). doi: 10.1099/ijsem.0.006183.
A Gram-stain-negative, facultative anaerobic, methylphosphonate-decomposing, motile by a polar flagellum and rod-shaped marine bacterium, designated S4B1, was isolated from the surface seawater collected from the Yongle Atoll (Xisha Islands, PR China). The pairwise alignment showed the highest sequence similarity of 97.5 and 96.6 % to subsp. 12_122_3T3 and HHS02, respectively. Phylogenetic analysis based on 16S rRNA gene and the phylogenomic analysis of single-copy genes showed that strain S4B1 belonged to the genus and formed a close branch with ZSDZ65. Growth of strain S4B1 occurred at 4-30 °C (optimum, 28 °C), at pH 6.0-8.0 (optimum, pH 7.0) and in the presence of 2-7 % (w/v) NaCl (optimum, 3 %). The predominant fatty acids (>10 %) were C16 : 0, iso-C and summed feature 3 (C 7 or/and C 6). The DNA G+C content of the assembled genomic sequence was 44.3 mol%. Average nucleotide identity (ANI) values between S4B1 and its reference species were lower than the threshold for species delineation (95-96 %), in which its highest ANI value with ZSDZ65 was 87.0 %. DNA-DNA hybridization further showed that strain S4B1 had less than 70 % similarity to its relatives. On the basis of the polyphasic evidence, strain S4B1 is proposed to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is S4B1 (=KCTC 92311=MCCC 1K06168).
一株革兰氏阴性、兼性厌氧、能分解甲基膦酸、通过极生鞭毛运动的杆状海洋细菌，被命名为 S4B1，从中国西沙群岛永乐环礁的表层海水中分离得到。序列比对表明，它与 subsp. 12_122_3T3 和 HHS02 的相似度最高，分别为 97.5%和 96.6%。基于 16S rRNA 基因的系统发育分析和单拷贝基因的系统基因组分析表明，S4B1 菌株属于 属，与 ZSDZ65 形成一个紧密的分支。S4B1 菌株的生长条件为 4-30°C（最佳温度为 28°C），pH 值为 6.0-8.0（最佳 pH 值为 7.0），盐度为 2-7%（w/v）（最佳盐度为 3%）。优势脂肪酸（>10%）为 C16:0、iso-C 和特征性脂肪酸 3（C7 或/和 C6）。组装基因组序列的 DNA G+C 含量为 44.3mol%。S4B1 与参考种之间的平均核苷酸同一性（ANI）值低于种间区分的阈值（95-96%），其中与 ZSDZ65 的最高 ANI 值为 87.0%。DNA-DNA 杂交进一步表明，S4B1 与亲缘关系较近的菌株的相似度低于 70%。基于多相证据，S4B1 菌株代表了 属的一个新种，建议命名为 sp. nov.。其模式菌株为 S4B1（=KCTC 92311=MCCC 1K06168）。
