Department of Microbiology, Faculty of Science, Kasetsart University, Chatuchak, Bangkok, 10900, Thailand.
Department of Microbiology, Faculty of Liberal Arts and Science, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom, 73140, Thailand.
J Antibiot (Tokyo). 2020 Jul;73(7):435-440. doi: 10.1038/s41429-020-0294-5. Epub 2020 Mar 5.
A novel actinobacterium, designated strain K1PN6, was isolated from soil sample collected in Kantulee peat swamp forest, Surat Thani province, Thailand. The morphological, chemotaxonomic, and phylogenetic characteristics were consistent with its classification in the genus Streptomyces. Based on 16S rRNA gene sequence analysis, strain K1PN6 showed highest similarity to Streptomyces phyllanthi PA1-07 (98.6 %), Streptomyces spongiae Sp080513SC-24 (98.3%) and Streptomyces adustus WH-9 (98.3%). The G + C content of the genomic DNA was 70.3 mol%. Digital DNA-DNA hybridization and average nucleotide identity values between the genome sequence of strain K1PN6 with S. phyllanthi TISTR 2346 (33.7 and 89.1%), S. spongiae NBRC 106415 (38.6 and 90.6%) and S. adustus NBRC 109810 (26.0 and 86.2%) were below the thresholds of 70 and 95-96% for prokaryotic conspecific assignation. Chemotaxonomic data revealed that strain K1PN6 possessed MK-9(H) (45%) and MK-9(H) (34%) as the predominant menaquinones. It contained LL-diaminopimelic acid as the diagnostic diamino acid and galactose, glucose, mannose, and ribose as whole-cell sugars. The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, two unidentified aminolipids, an unidentified phospholipid, and glycophospholipid. The predominant cellular fatty acids (>10%) were iso-C, C, anteiso-C, and iso-C. On the basis of these genotypic and phenotypic data, strain K1PN6 should be designated as a representative of a novel species of the genus Streptomyces, for which the name Streptomyces acidicola sp. nov. is proposed with the type strain K1PN6 (=TBRC 11341=NBRC 114304).
一株新的放线菌，命名为 K1PN6 菌株，从泰国素叻他尼省坎特利泥炭沼泽森林的土壤样本中分离出来。形态学、化学分类学和系统发育特征均与链霉菌属的分类一致。基于 16S rRNA 基因序列分析，菌株 K1PN6 与 Phyllanthi 链霉菌 PA1-07（98.6%）、Spongiae 链霉菌 Sp080513SC-24（98.3%）和 Adustus 链霉菌 WH-9（98.3%）的相似度最高。基因组 DNA 的 G+C 含量为 70.3mol%。K1PN6 菌株与 Phyllanthi 链霉菌 TISTR 2346（33.7%和 89.1%）、Spongiae 链霉菌 NBRC 106415（38.6%和 90.6%）和 Adustus 链霉菌 NBRC 109810（26.0%和 86.2%）的 DNA-DNA 杂交值和平均核苷酸同一性值均低于 70%和 95-96%的原核生物同型性认定阈值。化学分类学数据表明，K1PN6 菌株含有 MK-9(H)（45%）和 MK-9(H)（34%）作为主要的menaquinones。它含有 LL-二氨基庚二酸作为诊断二氨基庚二酸，以及半乳糖、葡萄糖、甘露糖和核糖作为全细胞糖。极性脂类由二磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、磷脂酰肌醇、磷脂酰肌醇甘露糖苷、两种未鉴定的氨基脂类、一种未鉴定的磷脂和糖脂组成。主要的细胞脂肪酸（>10%）为 iso-C、C、anteiso-C 和 iso-C。基于这些基因型和表型数据，K1PN6 菌株应被指定为链霉菌属的一个新种的代表，该新种被命名为 Strepromyces acidicola sp. nov.，其模式菌株为 K1PN6（=TBRC 11341=NBRC 114304）。
