Berckx Fede, Bandong Cyndi Mae, Wibberg Daniel, Kalinowski Jörn, Willemse Joost, Brachmann Andreas, Simbahan Jessica, Pawlowski Katharina
Department of Ecology, Environment and Plant Sciences, Stockholm University, 106 91 Stockholm, Sweden.
Institute of Biology, College of Science, University of the Philippines, Diliman, Quezon City, Philippines.
Int J Syst Evol Microbiol. 2022 Dec;72(12). doi: 10.1099/ijsem.0.005603.
An actinobacterial strain, CMB-FB, was isolated from surface-sterilized root nodules of a plant growing along Halsema Highway in the province of Benguet (Luzon, Philippines). The 16S rRNA gene sequence of CMB-FB showed high sequence similarity to those of the type strains of (99.4 %), (99.1 %), subsp. (99.0 %), and (98.6 %). The major menaquinones of CMB-FB were composed of MK-9(H), MK-9(H) and MK-9(H), and there was a minor contribution of MK-9(H). The polar lipid profile consisted of phosphatidylethanolamine, unidentified aminolipids and phospholipids, a glycophospholipid and four unidentified lipids. The diagnostic diamino acid of the peptidoglycan was -diaminopimelic acid. The major fatty acids were iso-C, anteiso-C and anteiso-C. The results of physiological analysis indicated that CMB-FB was mesophilic. The results of phylogenetic, genome-genome distance calculation and average nucleotide identity analysis indicated that the isolated strain represents the type strain of a novel species. On the basis of these results, strain CMB-FB (=DSM 112754=LMG 32457) is proposed as the type strain of the novel species sp. nov.
从菲律宾吕宋岛本格特省哈尔塞马公路沿线生长的一种植物经表面消毒的根瘤中分离出一株放线菌菌株CMB - FB。CMB - FB的16S rRNA基因序列与（99.4%）、（99.1%）、亚种（99.0%）和（98.6%）的模式菌株的序列具有高度相似性。CMB - FB的主要甲基萘醌由MK - 9(H)、MK - 9(H)和MK - 9(H)组成，MK - 9(H)有少量贡献。极性脂质谱由磷脂酰乙醇胺、未鉴定的氨基脂质和磷脂、一种糖脂和四种未鉴定的脂质组成。肽聚糖的诊断性二氨基酸为 - 二氨基庚二酸。主要脂肪酸为异-C、anteiso-C和anteiso-C。生理分析结果表明CMB - FB是嗜温菌。系统发育、全基因组距离计算和平均核苷酸同一性分析结果表明，分离出的菌株代表一个新物种的模式菌株。基于这些结果，菌株CMB - FB（=DSM 112754 = LMG 32457）被提议作为新物种新种的模式菌株。
