sp. nov., a novel streptomycete isolated from actinorhizal nodules of .

An actinobacterial strain, CMB-FB, was isolated from surface-sterilized root nodules of a plant growing along Halsema Highway in the province of Benguet (Luzon, Philippines). The 16S rRNA gene sequence of CMB-FB showed high sequence similarity to those of the type strains of (99.4 %), (99.1 %), subsp. (99.0 %), and (98.6 %). The major menaquinones of CMB-FB were composed of MK-9(H), MK-9(H) and MK-9(H), and there was a minor contribution of MK-9(H). The polar lipid profile consisted of phosphatidylethanolamine, unidentified aminolipids and phospholipids, a glycophospholipid and four unidentified lipids. The diagnostic diamino acid of the peptidoglycan was -diaminopimelic acid. The major fatty acids were iso-C, anteiso-C and anteiso-C. The results of physiological analysis indicated that CMB-FB was mesophilic. The results of phylogenetic, genome-genome distance calculation and average nucleotide identity analysis indicated that the isolated strain represents the type strain of a novel species. On the basis of these results, strain CMB-FB (=DSM 112754=LMG 32457) is proposed as the type strain of the novel species sp. nov.