Zhou Jinquan, Yang Cailing, Meng Qingfeng, Fu Shaobin
School of Pharmacy, Zunyi Medical University, Zunyi, People's Republic of China.
Department of Public Health, Zunyi Medical University, Zunyi, People's Republic of China; Centre of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai, Thailand.
Int J Med Mushrooms. 2023;25(2):49-54. doi: 10.1615/IntJMedMushrooms.2022046927.
Species of the genus Morchella are highly prized worldwide for their excellent flavor and high medicinal value. In recent years, artificial cultivations of medicinal fungi with many advantages have elicited great interest as a promising alternative to produce certain valuable metabolites. Therefore, the secondary metabolites of fermented M. importuna belonging to the black morel clade isolated from China were investigated. The strain was cultured in a fermentation tank in PDB liquid medium by two-step method. The mycelia and fermentation broth were extracted by ethyl acetate. The secondary metabolites were separated and purified by repeated silica gel column chromatography. Structures of compounds were determined by NMR data and references. One new natural compound (1) and six known compounds (2-7) were obtained. Compounds 1, 2, 4, and 5 were first isolated from genus Morchella and compounds 3, 6, and 7 are first isolated from species M. importuna.
羊肚菌属的物种因其出色的风味和高药用价值而在全球备受推崇。近年来，具有诸多优势的药用真菌人工栽培作为生产某些有价值代谢产物的一种有前景的替代方法引起了极大关注。因此，对从中国分离出的属于黑羊肚菌分支的进口羊肚菌发酵后的次生代谢产物进行了研究。该菌株通过两步法在PDB液体培养基的发酵罐中培养。用乙酸乙酯提取菌丝体和发酵液。通过反复硅胶柱色谱法分离和纯化次生代谢产物。通过核磁共振数据和参考文献确定化合物的结构。得到了一种新的天然化合物（1）和六种已知化合物（2 - 7）。化合物1、2、4和5首次从羊肚菌属中分离得到，化合物3、6和7首次从进口羊肚菌物种中分离得到。
