Engineering Research Centre of Industrial Microbiology, Ministry of Education, College of Life Sciences, Fujian Normal University, Fuzhou 350117, China.
Provincial University Key Laboratory of Cellular Stress Response and Metabolic Regulation, College of Life Sciences, Fujian Normal University, Fuzhou 350117, China.
Molecules. 2023 Jun 14;28(12):4760. doi: 10.3390/molecules28124760.
In recent years, wild morel mushroom species have begun to be widely cultivated in China due to their high edible and medicinal values. To parse the medicinal ingredients, we employed the technique of liquid-submerged fermentation to investigate the secondary metabolites of . Two new natural isobenzofuranone derivatives (-) and one new orsellinaldehyde derivative (), together with seven known compounds, including one -orsellinaldehyde (), phenylacetic acid (), benzoic acid (), 4-hydroxy-phenylacetic acid (), 3,5-dihydroxybenzoic acid (), ,'-pentane-1,5-diyldiacetamide (), and 1H-pyrrole-2-carboxylic acid (), were obtained from the fermented broth of . Their structures were determined according to the data of NMR, HR Q-TOF MS, IR, UV, optical activity, and single-crystal X-ray crystallography. TLC-bioautography displayed that these compounds possess significant antioxidant activity with the half DPPH free radical scavenging concentration of 1.79 (), 4.10 (), 4.28 (), 2.45 (), 4.40 (), 1.73 (), and 6.00 () mM. The experimental results would shed light on the medicinal value of for its abundant antioxidants.
近年来，由于野生羊肚菌具有较高的食用和药用价值，中国开始广泛栽培野生羊肚菌品种。为了解析其药用成分，我们采用液体深层发酵技术研究了. 的次生代谢产物。从发酵液中分离得到了两个新的天然异苯并呋喃酮衍生物（(-)和（）和一个新的奥索利醛衍生物（），以及 7 个已知化合物，包括一个奥索利醛（）、苯乙酸（）、苯甲酸（）、4-羟基苯乙酸（）、3,5-二羟基苯甲酸（）、,-戊烷-1,5-二基二乙酰胺（）和 1H-吡咯-2-羧酸（）。根据 NMR、HR Q-TOF MS、IR、UV、旋光和单晶 X 射线衍射数据确定了它们的结构。TLC 生物自显影显示，这些化合物具有显著的抗氧化活性，其半 DPPH 自由基清除浓度分别为 1.79（）、4.10（）、4.28（）、2.45（）、4.40（）、1.73（）和 6.00（）mM。实验结果表明，丰富的抗氧化剂赋予了. 较高的药用价值。
