UWA Dental School, The University of Western Australia, Perth, Western Australia, Australia.
School of Molecular Sciences, The University of Western Australia, Perth, Western Australia, Australia.
Int Dent J. 2023 Jun;73(3):354-361. doi: 10.1016/j.identj.2023.01.002. Epub 2023 Feb 6.
This study aimed to synthesise a drug-delivery system based on a porous polymer hydrogel, with antimicrobial properties against Porphyromonas gingivalis and potential to be used in tissue regeneration.
2-Hydroxyethyl methacrylate monomers were polymerised using thermal and photoactivation in the presence of silver nitrate (AgNO) and/or chlorhexidine digluconate. Poly-2-hydroxyethyl methacrylate (pHEMA) hydrogels containing silver nanoparticles (AgNPs) and/or 0.12% chlorhexidine (CHX) were produced and characterised using cryo-SEM and confocal microscopy. Hydrogel degradation and leaching of AgNP were tested for 1.5 months. The antimicrobial properties were tested against P. gingivalis using broth culture system and disk diffusion tests.
Our methodology manufactured porous polymeric hydrogels doped with AgNPs and CHX. Hydrogels showed a successful delivery of CHX and sustainable release of AgNPs in a steady hydrogel degradation rate determined based on the weight loss of samples. Hydrogels with AgNPs or CHX had a significant antimicrobial effect against P. gingivalis, with CHX-hydrogels exhibiting a stronger effect than AgNP-hydrogels in the short-term assessment. AgNP-CHX hydrogels showed a compounded antimicrobial effect, whereas control hydrogels containing neither AgNPs nor CHX had no influence on bacterial growth (P < .05).
The dual-cured pHEMA hydrogel loaded with antimicrobial agents proved to be an efficient drug-delivery system against periodontopathogens, with the potential to be used as a scaffold for tissue regeneration.
本研究旨在合成一种基于多孔聚合物水凝胶的药物递送系统，该系统具有抗牙龈卟啉单胞菌的抗菌性能，并具有用于组织再生的潜力。
使用热和光在硝酸银（AgNO）和/或葡萄糖酸氯己定存在的情况下聚合 2-羟乙基甲基丙烯酸酯单体。生产并使用冷冻扫描电子显微镜和共聚焦显微镜对含有银纳米颗粒（AgNPs）和/或 0.12%葡萄糖酸氯己定（CHX）的聚-2-羟乙基甲基丙烯酸酯（pHEMA）水凝胶进行了表征。测试了水凝胶的降解和 AgNP 的浸出作用，时间为 1.5 个月。使用肉汤培养系统和圆盘扩散试验测试了针对 P. gingivalis 的抗菌性能。
我们的方法制造了掺杂有 AgNPs 和 CHX 的多孔聚合物水凝胶。水凝胶显示出成功地输送 CHX 和 AgNPs 的可持续释放，AgNP 的释放与根据样品重量损失确定的稳定水凝胶降解率相一致。含有 AgNPs 或 CHX 的水凝胶对 P. gingivalis 具有显著的抗菌作用，在短期评估中，CHX-水凝胶的作用比 AgNP-水凝胶更强。AgNP-CHX 水凝胶显示出复合抗菌作用，而既不含有 AgNPs 也不含有 CHX 的对照水凝胶对细菌生长没有影响（P<0.05）。
负载抗菌剂的双固化 pHEMA 水凝胶被证明是一种针对牙周病原体的有效药物递送系统，具有作为组织再生支架的潜力。
