Jasso-Díaz G, Torres-Hernández G, Zamilpa A, Becerril-Pérez C M, Ramírez-Bribiesca J E, Hernández-Mendo O, Sánchez-Arroyo H, Olmedo-Juárez A, González-Cortazar M, Mendoza-De Gives P
Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad, INIFAP, Boulevar Paseo Cuauhnauac No. 8534, Col. Progreso, Jiutepec, Morelos, CP 62550, México.
Colegio de Postgraduados, Campus Montecillo, Texcoco, Estado de México. CP 56230, México.
Helminthologia. 2022 May 3;59(1):46-54. doi: 10.2478/helm-2022-0001. eCollection 2022 Mar.
The ovicidal and larvicidal effect of a full aqueous extract (FE) and two phases: an aqueous (Aq-Ph) and an ethyl acetate (EtOAc-Ph) from () stems and leaves against () were assessed. The egg hatching inhibition (EHI) assay and larval mortality (LM) test were performed by triplicate in 96-well micro-titration plates (n=4 wells). The FE against eggs and larvae was assessed at 1.25, 2.5, 5, 10 and 20 mg/mL; and 30, 60, 90, 120,150 and 200 mg/ mL, respectively. The ovicidal effect of Aq-F and EtOAc-F was assessed at 1.25, 2.5, 5, 10 and 20 mg/mL. Plates were incubated at 28 °C for 48 (ovicidal assay) and 72 h (larvicidal assay). The EHI results were considered based on the mean number of eggs hatching failure after 48 h exposure. The LM was recorded after 72 h exposure to the phases and expressed as mortality percentage. The FE caused 96 and 100% EHI at 10 and 20 mg/mL, respectively; meanwhile, 74% LM was recorded at 200 mg/mL (p<0.05). The Aq-Ph showed 78.5% EHI at 2.5 mg/mL. Likewise, the EtOAc-Ph caused 100% EHI in almost all concentrations. Eighteen compounds including alkaloids, cumarins, triterpens, flavonoids, tannins, saponins and sterols were identified by GC-MS analysis. The results indicate that the aqueous extract from possesses bioactive compounds with nematocidal activity against ; mainly in the EtOAc-Ph. Further studies should be performed to elucidate those compounds searching for alternative methods of control of the sheep haemonchosis.
评估了来自（）茎叶的全水提取物（FE）以及两个相：水相（Aq-Ph）和乙酸乙酯相（EtOAc-Ph）对（）的杀卵和杀幼虫效果。在96孔微量滴定板中进行三次重复的卵孵化抑制（EHI）试验和幼虫死亡率（LM）测试（n = 4孔）。分别以1.25、2.5、5、10和20 mg/mL评估FE对卵和幼虫的作用；以及30、60、90、120、150和200 mg/mL。以1.25、2.5、5、10和20 mg/mL评估Aq-F和EtOAc-F的杀卵效果。将平板在28°C下孵育48小时（杀卵试验）和72小时（杀幼虫试验）。基于暴露48小时后未孵化成功的卵的平均数量来考虑EHI结果。在暴露于各相72小时后记录LM，并以死亡率百分比表示。FE在10和20 mg/mL时分别引起96%和100%的EHI；同时，在200 mg/mL时记录到74%的LM（p<0.05）。Aq-Ph在2.5 mg/mL时显示78.5%的EHI。同样，EtOAc-Ph在几乎所有浓度下都引起100%的EHI。通过GC-MS分析鉴定出18种化合物，包括生物碱、香豆素、三萜、黄酮类、单宁、皂苷和甾醇。结果表明，来自（）的水提取物含有对（）具有杀线虫活性的生物活性化合物；主要存在于EtOAc-Ph中。应进行进一步研究以阐明这些化合物，寻找控制绵羊血矛线虫病的替代方法。
