Centro de Investigación en Biotecnología, Universidad Autónoma de Morelos, Doctorado en Ciencias Naturales (CEIB-UAEM) Av. Universidad 1001 Col. Chamilpa, Cuernavaca, Morelos CP 62209, Mexico.
Universidad Autónoma de Guerrero, Av. Javier Méndez Aponte No. 1. Chilpancingo, Guerrero CP 39000, Mexico.
Biomolecules. 2020 May 15;10(5):773. doi: 10.3390/biom10050773.
affect small ruminants all over the world. Anthelmintics cause resistance, contamination, and a risk of public health. is a plant used as a home remedy against many diseases in Mexico. This study arose from a preliminary study where a hydroalcoholic extract ( showed anthelmintic activity () against . Searching for bioactive compounds () with high , the was fractioned obtaining an aqueous () and an ethyl acetate fraction (), and a flavonoid with identified as isorhamnetin was obtained from . Both fractions were in vitro assessed by the egg hatch test () and larval mortality () assays. The obtained from were characterised by NMR analysis. The highest were recorded with , resulting in 100% and 80.45% at 0.75 and 30 mg/mL, respectively. Alterations in eggs and larvae attributed to isorhamnetin were recorded by environmental scanning electron microscopy, confocal laser scanning and by high-resolution digital-coupled camera. This flavonoid caused 100% at 0.07 mg/mL after 48 h and 100% at 7.5 mg/mL after 72 h exposure. Isorhamnetin has promising potential as an anthelmintic against sheep haemonchosis.
该植物被用作墨西哥治疗许多疾病的家庭偏方。这项研究源于一项初步研究，该研究表明，一种水醇提取物（）对（）具有驱虫活性（）。为了寻找具有高生物利用度（）的生物活性化合物（），对进行了分段，得到了水（）和乙酸乙酯（）部分，并从中获得了一种具有驱虫活性的黄酮类化合物（），鉴定为异鼠李素。通过卵孵化试验（）和幼虫死亡率（）试验对两种部分进行了体外评估。从获得的进行了 NMR 分析。在 0.75 和 30 mg/mL 时， 获得的显示出最高的驱虫活性（），分别导致 100%和 80.45%的孵化抑制率和幼虫死亡率。环境扫描电子显微镜、共聚焦激光扫描和高分辨率数字耦合相机记录了异鼠李素对卵和幼虫的影响。该黄酮类化合物在 48 小时时，在 0.07 mg/mL 时即可达到 100%的驱虫效果，在 72 小时时，在 7.5 mg/mL 时即可达到 100%的驱虫效果。异鼠李素作为抗绵羊血矛线虫病的驱虫药具有很大的潜力。
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