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具有均匀排列纳米孔结构的超薄膜过滤器，用于细胞外囊泡的纳米级分离且无滤饼形成。

Ultra-thin membrane filter with a uniformly arrayed nanopore structure for nanoscale separation of extracellular vesicles without cake formation.

作者信息

Kim Daesan, Lee Jaehyuk, Kim Boyoung, Shin Yujin, Park Jinhong, Kim Uijoo, Lee Minbaek, Kim Sang Bum, Kim Sunghoon

机构信息

Medicinal Bioconvergence Research Center, Institute for Artificial Intelligence and Biomedical Research, Gangnam Severance Hospital, Yonsei University Incheon 21983 Korea

R&D Center, Metapore Co., Ltd, Advanced Institutes of Convergence Technology 8F Suwon 16229 Korea.

出版信息

Nanoscale Adv. 2022 Nov 22;5(3):640-649. doi: 10.1039/d2na00227b. eCollection 2023 Jan 31.

DOI:10.1039/d2na00227b
PMID:36756507
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9890561/
Abstract

Extracellular vesicles (EVs) have emerged as vehicles that mediate diverse cell-cell communication. However, in-depth understanding of these vesicles is hampered by a lack of a reliable isolation method to separate different types of EVs with high levels of integrity and purity. Here, we developed a nanoporous and ultra-thin membrane structure (NUTS) that warrants the size-based isolation of EVs without cake formation, minimizing the sample loss during the filtration process. By utilizing the micro-electro-mechanical systems (MEMS) technique, we could also control the pore size in nanoscale. We validated the performance of this membrane to separate EVs according to their size range.

摘要

细胞外囊泡（EVs）已成为介导多种细胞间通讯的载体。然而，由于缺乏一种可靠的分离方法来分离具有高完整性和纯度的不同类型的细胞外囊泡，对这些囊泡的深入了解受到了阻碍。在此，我们开发了一种纳米多孔超薄膜结构（NUTS），该结构可确保基于尺寸分离细胞外囊泡且不会形成滤饼，从而将过滤过程中的样品损失降至最低。通过利用微机电系统（MEMS）技术，我们还能够在纳米尺度上控制孔径。我们验证了这种膜根据细胞外囊泡大小范围进行分离的性能。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d6d/9890561/bc006ef5570f/d2na00227b-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d6d/9890561/c635afecc11a/d2na00227b-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d6d/9890561/be50c22ebb85/d2na00227b-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d6d/9890561/e6d2cd07b7bb/d2na00227b-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d6d/9890561/329484be4ca3/d2na00227b-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d6d/9890561/bc006ef5570f/d2na00227b-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d6d/9890561/c635afecc11a/d2na00227b-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d6d/9890561/be50c22ebb85/d2na00227b-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d6d/9890561/e6d2cd07b7bb/d2na00227b-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d6d/9890561/329484be4ca3/d2na00227b-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d6d/9890561/bc006ef5570f/d2na00227b-f5.jpg

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