Rosenblad Magnus Alm, Larsson Ellen, Walker Arttapon, Thongklang Naritsada, Wurzbacher Christian, Nilsson R Henrik
Department of Chemistry and Molecular Biology, National Infrastructure of Bioinformatics (NBIS), Lundberg laboratory, University of Gothenburg, Gothenburg, Sweden.
Gothenburg Global Biodiversity Centre, Department of Biological and Environmental Sciences, University of Gothenburg, Box 461, 405 30 Gothenburg, Sweden.
MycoKeys. 2022 Jun 30;90:203-213. doi: 10.3897/mycokeys.90.84866. eCollection 2022.
Non-coding RNA (ncRNA) genes play important, but incompletely understood, roles in various cellular processes, notably translation and gene regulation. A recent report on the detection of the ncRNA Signal Recognition Particle gene in the nuclear ribosomal internal transcribed spacer region of several species of three genera of ectomycorrhizal basidiomycetes prompted a more thorough bioinformatics search for additional ncRNA genes in the full fungal ribosomal operon. This study reports on the detection of three ncRNA genes hitherto not known from the fungal ribosomal region: nuclear RNase P RNA, RNase MRP RNA, and a possible snoRNA U14 in a total of five species of and . We verified their presence through resequencing of independent specimens. Two completed genomes were found to lack these ncRNAs elsewhere than in the ribosomal operon, suggesting that these are functional genes. It seems clear that ncRNA genes play a larger role in fungal ribosomal genetics than hitherto thought.
非编码RNA（ncRNA）基因在各种细胞过程中发挥着重要但尚未完全理解的作用，尤其是在翻译和基因调控方面。最近一份关于在几种外生菌根担子菌三个属的核核糖体内部转录间隔区检测到ncRNA信号识别颗粒基因的报告，促使人们进行更全面的生物信息学搜索，以在完整的真菌核糖体操纵子中寻找更多的ncRNA基因。本研究报告了在总共五个物种中检测到三个迄今在真菌核糖体区域未知的ncRNA基因：核核糖核酸酶P RNA、核糖核酸酶MRP RNA和一种可能的小核仁RNA U14。我们通过对独立样本重新测序验证了它们的存在。发现两个完整的基因组在核糖体操纵子之外的其他地方缺乏这些ncRNA，这表明它们是功能基因。很明显，ncRNA基因在真菌核糖体遗传学中发挥的作用比迄今认为的要大。
