Ogrodowicz Piotr, Mikołajczak Krzysztof, Kempa Michał, Mokrzycka Monika, Krajewski Paweł, Kuczyńska Anetta
Institute of Plant Genetics, Polish Academy of Sciences, Poznan, Poland.
Front Plant Sci. 2023 Jan 24;14:1077631. doi: 10.3389/fpls.2023.1077631. eCollection 2023.
The root system is a key component for plant survival and productivity. In particular, under stress conditions, developing plants with a better root architecture can ensure productivity. The objectives of this study were to investigate the phenotypic variation of selected root- and yield-related traits in a diverse panel of spring barley genotypes. By performing a genome-wide association study (GWAS), we identified several associations underlying the variations occurring in root- and yield-related traits in response to natural variations in soil moisture. Here, we report the results of the GWAS based on both individual single-nucleotide polymorphism markers and linkage disequilibrium (LD) blocks of markers for 11 phenotypic traits related to plant morphology, grain quality, and root system in a group of spring barley accessions grown under field conditions. We also evaluated the root structure of these accessions by using a nondestructive method based on electrical capacitance. The results showed the importance of two LD-based blocks on chromosomes 2H and 7H in the expression of root architecture and yield-related traits. Our results revealed the importance of the region on the short arm of chromosome 2H in the expression of root- and yield-related traits. This study emphasized the pleiotropic effect of this region with respect to heading time and other important agronomic traits, including root architecture. Furthermore, this investigation provides new insights into the roles played by root traits in the yield performance of barley plants grown under natural conditions with daily variations in soil moisture content.
根系是植物生存和生产力的关键组成部分。特别是在胁迫条件下，培育具有更好根系结构的植物能够确保产量。本研究的目的是调查一组春大麦基因型中与根系和产量相关的选定性状的表型变异。通过进行全基因组关联研究（GWAS），我们确定了土壤水分自然变异响应下根系和产量相关性状变异背后的若干关联。在此，我们报告了基于个体单核苷酸多态性标记以及标记的连锁不平衡（LD）块对一组在田间条件下生长的春大麦种质中与植物形态、籽粒品质和根系相关的11个表型性状进行GWAS的结果。我们还使用基于电容的无损方法评估了这些种质的根系结构。结果表明2H和7H染色体上两个基于LD的块在根系结构和产量相关性状表达中的重要性。我们的结果揭示了2H染色体短臂上的区域在根系和产量相关性状表达中的重要性。本研究强调了该区域对抽穗期和其他重要农艺性状（包括根系结构）的多效性作用。此外，本研究为在土壤水分含量每日变化的自然条件下生长的大麦植株根系性状对产量表现所起的作用提供了新的见解。
