Yuan Yuxiang, Qin Liuyue, Su Henan, Yang Shuangjuan, Wei Xiaochun, Wang Zhiyong, Zhao Yanyan, Li Lin, Liu Honglei, Tian Baoming, Zhang Xiaowei
Institute of Horticulture, Henan Academy of Agricultural Sciences, Graduate T&R Base of Zhengzhou University, Zhengzhou, China.
School of Life Sciences, Zhengzhou University, Zhengzhou, China.
Front Plant Sci. 2021 Aug 10;12:650252. doi: 10.3389/fpls.2021.650252. eCollection 2021.
Clubroot, caused by the soil-borne protist , is one of the most destructive diseases of Chinese cabbage worldwide. However, the clubroot resistance mechanisms remain unclear. In this study, in both clubroot-resistant (DH40R) and clubroot-susceptible (DH199S) Chinese cabbage lines, the primary (root hair infection) and secondary (cortical infection) infection stages started 2 and 5 days after inoculation (dai), respectively. With the extension of the infection time, cortical infection was blocked and complete resistance was observed in DH40R, while disease scales of 1, 2, and 3 were observed at 8, 13, and 22 dai in DH199S. Transcriptome analysis at 0, 2, 5, 8, 13, and 22 dai identified 5,750 relative DEGs (rDEGs) between DH40R and DH199S. The results indicated that genes associated with auxin, PR, disease resistance proteins, oxidative stress, and and transcription factors were involved in clubroot resistance regulation. In addition, weighted gene coexpression network analysis (WGCNA) identified three of the modules whose functions were highly associated with clubroot-resistant, including ten hub genes related to clubroot resistance (, , , , , , two , , and ). These results provide valuable information for better understanding the molecular regulatory mechanism of Chinese cabbage clubroot resistance.
由土壤传播的原生生物引起的根肿病是全球范围内大白菜最具毁灭性的病害之一。然而，根肿病的抗性机制仍不清楚。在本研究中，在抗根肿病（DH40R）和感根肿病（DH199S）的大白菜品系中，初次感染阶段（根毛感染）和二次感染阶段（皮层感染）分别在接种后2天和5天开始。随着感染时间的延长，皮层感染受到阻碍，在DH40R中观察到完全抗性，而在DH199S中，在接种后8天、13天和22天观察到病情等级为1、2和3。在接种后0天、2天、5天、8天、13天和22天进行的转录组分析确定了DH40R和DH199S之间5750个相对差异表达基因（rDEG）。结果表明，与生长素、病程相关蛋白、抗病蛋白、氧化应激以及转录因子相关的基因参与了根肿病抗性调控。此外，加权基因共表达网络分析（WGCNA）确定了三个功能与抗根肿病高度相关的模块，包括十个与根肿病抗性相关的枢纽基因（、、、、、、两个、、和）。这些结果为更好地理解大白菜根肿病抗性的分子调控机制提供了有价值的信息。
