Zhao Weisong, Wang Peipei, Dong Lihong, Li Shezeng, Lu Xiuyun, Zhang Xiaoyun, Su Zhenhe, Guo Qinggang, Ma Ping
Integrated Pest Management Innovation Center of Hebei Province, Key Laboratory of IPM on Crops in Northern Region of North China, Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences, Ministry of Agriculture and Rural Affairs, Baoding, China.
Front Bioeng Biotechnol. 2023 Jan 24;11:1115656. doi: 10.3389/fbioe.2023.1115656. eCollection 2023.
Cotton verticillium wilt (CVW) represented a typical plant soil-borne disease and resulted in widespread economic losses in cotton production. However, the effect of broccoli residues (BR) on verticillium wilt of spring-sowing-cotton was not clear. We investigated the effects of BR on CVW, microbial communities structure and function in rhizosphere of two cotton cultivars with different CVW resistance using amplicon sequencing methods. Results showed that control effects of BR on CVW of susceptible cultivar (cv. EJ-1) and resistant cultivar (cv. J863) were 58.49% and 85.96%, and the populations of decreased by 14.31% and 34.19%, respectively. The bacterial diversity indices significantly increased in BR treatment, while fungal diversity indices significantly decreased. In terms of microbial community composition, the abilities to recruit bacteria and fungi were enhanced in BR treatment, including , , , , , , , and , , , and . However, the relative abundances of , , , , , and were opposite tendency between cultivars in BR treatment. According to PICRUSt analysis, functional profiles prediction showed that significant shifts in metabolic functions impacting KEGG pathways of BR treatment were related to metabolism and biosynthesis. FUNGuild analysis indicated that BR treatment altered the relative abundances of fungal trophic modes. The results of this study demonstrated that BR treatment decreased the populations of in soil, increased bacterial diversity, decreased fungal diversity, changed the microbial community structure and function, and increased the abundances of beneficial microorganisms.
棉花黄萎病是一种典型的植物土传病害，给棉花生产造成了广泛的经济损失。然而，西兰花残体（BR）对春播棉花黄萎病的影响尚不清楚。我们采用扩增子测序方法，研究了BR对两个不同棉花黄萎病抗性品种根际黄萎病菌、微生物群落结构和功能的影响。结果表明，BR对感病品种（EJ-1）和抗病品种（J863）黄萎病的防治效果分别为58.49%和85.96%，其数量分别减少了14.31%和34.19%。BR处理下细菌多样性指数显著增加，而真菌多样性指数显著降低。在微生物群落组成方面，BR处理增强了招募细菌和真菌的能力，包括、、、、、、、以及、、、和。然而，BR处理下、、、、、和的相对丰度在不同品种间呈相反趋势。根据PICRUSt分析，功能谱预测表明，BR处理影响KEGG通路的代谢功能发生显著变化，与代谢和生物合成有关。FUNGuild分析表明，BR处理改变了真菌营养模式的相对丰度。本研究结果表明，BR处理降低了土壤中的数量，增加了细菌多样性，降低了真菌多样性，改变了微生物群落结构和功能，并增加了有益微生物的丰度。
