Hu Kai, Zhang Nian-Nian, Yang Zai-Hua
Guizhou Academy of Forestry, Guiyang, China Guizhou Academy of Forestry Guiyang China.
Biodivers Data J. 2021 Oct 25;9:e69196. doi: 10.3897/BDJ.9.e69196. eCollection 2021.
The mitogenome of (Chevrolat, 1878) was sequenced and annotated to better identify and related species. The mitogenome is 18,680 bp in length, includes the 37 typical mitochondrial genes (13 protein-coding genes, two ribosomal RNA genes and 22 transfer RNA genes) and two control regions (total length: 3,879 bp). Mitogenome organisation, nucleotide composition and codon usage are similar to the previously sequenced mitogenomes. All 13 protein-coding genes use ATN or TTG as start codon and end with TAA/G or incomplete stop codons (single T-). Twenty-one transfer RNA genes have the typical clover-leaf structure, while the dihydrouridine (DHU) arm of trnS1 is missing. In mitogenomes, the size of the control region is highly variable. Both ML and BI analyses, based on the 13 PCGs and two rRNAs from six species of Curculioninae, strongly supported the monophyly of . In , the relationships amongst included species were inferred as (( + . sp.) + ( + )), with and sp. forming a clade (BS = 100; PP = 1).
对（Chevrolat，1878年）的线粒体基因组进行了测序和注释，以更好地识别该物种及相关物种。线粒体基因组长度为18,680 bp，包括37个典型的线粒体基因（13个蛋白质编码基因、2个核糖体RNA基因和22个转移RNA基因）以及两个控制区（总长度：3,879 bp）。线粒体基因组的组织、核苷酸组成和密码子使用情况与先前测序的线粒体基因组相似。所有13个蛋白质编码基因均以ATN或TTG作为起始密码子，并以TAA/G或不完整的终止密码子（单个T -）结束。21个转移RNA基因具有典型的三叶草结构，而trnS1的二氢尿嘧啶（DHU）臂缺失。在象甲科线粒体基因组中，控制区的大小高度可变。基于象甲亚科六个物种的13个PCG和两个rRNA进行的最大似然法（ML）和贝叶斯推断（BI）分析均有力地支持了该物种的单系性。在该物种中，所包含物种之间的关系推断为（（ + . sp.） + （ + ）），其中 和 sp. 形成一个分支（BS = 100；PP = 1）。
