Roberto João Paulo de Lacerda, Limeira Clécio Henrique, Araújo Júnior João Pessoa, Malossi Camila Dantas, Ullmann Leila Sabrina, Silva Maria Luana Cristiny Rodrigues, Dantas Antônio Flavio Medeiros, do Nascimento Maria Jussara Rodrigues, de Azevedo Sergio Santos, Alves Clebert José
Federal University of Campina Grande, Post-Graduate Program in Animal Science and Health, Patos, PB, Brazil.
São Paulo of University, São Paulo, Brazil.
Tuberculosis (Edinb). 2023 Mar;139:102319. doi: 10.1016/j.tube.2023.102319. Epub 2023 Feb 3.
The objective of this study was to report the occurrence of Mycobacterium avium subspecies paratuberculosis (MAP) in dairy goats, via description of their clinical presentation, histopathological findings, and molecular identification of the infectious agent. Screening was performed using IS900 real-time PCR (qPCR) in milk samples from 179 properties in the semiarid of Northeast region of Brazil. Pooled milk samples from all lactating goats from processing plants were submitted to molecular diagnosis. One property had a positive result at qPCR. The production unit which had the positive sample for MAP was located, and an on-site visit to this property was performed to collect individual milk samples, seven of which tested MAP positive by IS900 qPCR. With permission from the owner, two goats (Animal 1 was positive and Animal 2 was negative on first qPCR for MAP) were acquired and euthanized. Animals 1 and 2 had milk and portions of the duodenum, ileum, colon, and mesenteric lymph nodes positive at qPCR for MAP. Animal 1 also had MAP DNA detected in part of the jejunum and cecum. In animal 2, the ileocecal valve tested positive. MAP was not detected in the blood or feces of either animal; however; it was confirmed for the association of clinical findings, histopathology, and qPCR. The gene IS900 from the positive samples were sequenced and showed a 99% similarity with MAP. The MAP was identified for the first time in the goat milk and tissues in the semiarid region of Northeast Brazil.
本研究的目的是通过描述奶山羊的临床表现、组织病理学发现以及对感染病原体的分子鉴定，报告副结核分枝杆菌（MAP）在奶山羊中的发生情况。使用IS900实时荧光定量PCR（qPCR）对巴西东北部半干旱地区179个养殖场的牛奶样本进行筛查。将加工厂所有泌乳山羊的混合牛奶样本进行分子诊断。有一个养殖场的qPCR结果呈阳性。确定了MAP检测呈阳性样本的生产单位，并对该养殖场进行实地考察以采集个体牛奶样本，其中7份样本经IS900 qPCR检测MAP呈阳性。经养殖场主许可，购买了两只山羊（动物1首次qPCR检测MAP呈阳性，动物2呈阴性）并实施安乐死。动物1和动物2的牛奶以及十二指肠、回肠、结肠和肠系膜淋巴结的部分样本经qPCR检测MAP呈阳性。动物1的空肠和盲肠部分也检测到MAP DNA。动物2的回盲瓣检测呈阳性。两只动物的血液或粪便中均未检测到MAP；然而，通过临床发现、组织病理学和qPCR证实了它们之间的关联。对阳性样本的IS900基因进行测序，结果显示与MAP的相似度为99%。在巴西东北部半干旱地区的山羊奶和组织中首次鉴定出MAP。
