Muñoz Jose, Oliver-De La Cruz Jorge, Forte Giancarlo, Pumera Martin
Future Energy and Innovation Laboratory, Central European Institute of Technology, Brno University of Technology, Purkyňova 123, 61200, Brno, Czech Republic.
Center for Translational Medicine (CTM), St. Anne's University Hospital, International Clinical Research Centre (FNUSA-ICRC), 62500, Brno, Czech Republic.
Biosens Bioelectron. 2023 Apr 15;226:115113. doi: 10.1016/j.bios.2023.115113. Epub 2023 Feb 3.
This work examines the suitability of graphene-based 3D-printed nanocomposite bioelectronics as innovative systems to in situ monitor and evaluate both breast cancer cell adhesion and the chemosensitivity of anti-cancer drugs. With this aim, 3D-printed nanocomposite graphene electrodes (3D-nGEs) -made of a commercially available graphene/polylactic acid filament- have been covalently biofunctionalized with an extracellular matrix protein (i.e., fibronectin) by exploiting the carbon reactivity of 3D-nGEs. The specificity and selectivity of the developed electrochemical system to monitor breast cancer cell adhesion has been tested via electrochemical impedance spectroscopy (EIS). Importantly, the resulting 3D-printed bioelectronic system displayed excellent accuracy for the rapid screening of anti-cancer drugs, which exactly corresponded with the results achieved by the standard optical method, while having the advantage of employing a label-free approach. In light of the current state-of-the-art in the field, this proof-of-concept connects electronics to biological systems within 3D printing technology, providing the bases for the sustainable and cost-effective manufacturing of graphene-based 3D-printed nanocomposite bioelectronics to simulate in vivo microenvironments using in situ and real time electronic output signals.
这项工作研究了基于石墨烯的3D打印纳米复合生物电子学作为创新系统用于原位监测和评估乳腺癌细胞粘附以及抗癌药物化学敏感性的适用性。为此，由市售石墨烯/聚乳酸长丝制成的3D打印纳米复合石墨烯电极（3D-nGEs）通过利用3D-nGEs的碳反应性与细胞外基质蛋白（即纤连蛋白）进行了共价生物功能化。已通过电化学阻抗谱（EIS）测试了所开发的电化学系统监测乳腺癌细胞粘附的特异性和选择性。重要的是，所得的3D打印生物电子系统在快速筛选抗癌药物方面显示出优异的准确性，这与标准光学方法所取得的结果完全一致，同时具有采用无标记方法的优势。鉴于该领域的当前技术水平，这一概念验证将电子学与3D打印技术中的生物系统相连接，为基于石墨烯的3D打印纳米复合生物电子学的可持续且经济高效的制造提供了基础，以便使用原位和实时电子输出信号模拟体内微环境。
