Ecology and Environmental Remediation Laboratory, Department of Botany, Gauhati University, Guwahati, 781014, Assam, India.
Ecology and Environmental Remediation Laboratory, Department of Botany, Gauhati University, Guwahati, 781014, Assam, India.
Chemosphere. 2023 Apr;321:138102. doi: 10.1016/j.chemosphere.2023.138102. Epub 2023 Feb 8.
Anthracene biodegradation potential has been studied in liquid culture and soil microcosm environment by employing green synthesized TiO nanoparticles (NPs) and Alcaligenes faecalis HP8. The bacterium was isolated from crude oil contaminated soil, while TiO nanoparticles were synthesized using Paenibacillus sp. HD1PAH and Cyperus brevifolius which have PAHs remediation abilities. The dual application of TiO nanoparticles and Alcaligenes faecalis HP8 decreases anthracene concentration up to 21.3% in liquid at the end of 7 days and 37.9% in the soil treatments after completion of 30 days. Besides, the GC-MS analysis revealed production of five metabolites including 1,2-anthracenedihydrodiol; 6,7-benzocoumarin; 3-hydroxy-2-naphthoic acid; salicylic acid and 9,10-anthraquinone at different time interval of the treatments. Anthracene degradation pathway confirms the breakdown of three ring anthracene to one ring salicylic acid. Additionally, soil dehydrogenase, urease, alkaline phosphatase, catalase and amylase activities increased up to 4.09 folds, 8.6 folds, 4.4 folds, 3.6 folds and 2.1 folds respectively after the combined treatments of TiO nanoparticles and Alcaligenes faecalis HP8. The bacterial biomass and residual anthracene concentration were found to be negatively correlated. Finally, the study brings into light a novel anthracene biodegradation pathway and provides a new dimension in nano assisted bacterial remediation.
采用绿色合成的 TiO2 纳米粒子（NPs）和产碱杆菌（Alcaligenes faecalis）HP8 在液体培养和土壤微宇宙环境中研究了蒽的生物降解潜力。该细菌是从受原油污染的土壤中分离出来的，而 TiO2 纳米粒子是使用对多环芳烃（PAHs）具有修复能力的鞘氨醇单胞菌（Paenibacillus sp.）HD1PAH 和香蒲（Cyperus brevifolius）合成的。TiO2 纳米粒子和产碱杆菌（Alcaligenes faecalis）HP8 的双重应用可将液体中蒽的浓度在 7 天结束时降低 21.3%，在 30 天完成后土壤处理中降低 37.9%。此外，GC-MS 分析表明，在处理的不同时间间隔内产生了五种代谢物，包括 1,2-蒽二氢二醇；6,7-苯并香豆素；3-羟基-2-萘酸；水杨酸和 9,10-蒽醌。蒽降解途径证实了三环蒽分解为单环水杨酸。此外，在 TiO2 纳米粒子和产碱杆菌（Alcaligenes faecalis）HP8 的联合处理后，土壤脱氢酶、脲酶、碱性磷酸酶、过氧化氢酶和淀粉酶活性分别提高了 4.09 倍、8.6 倍、4.4 倍、3.6 倍和 2.1 倍。细菌生物量和残留蒽浓度呈负相关。最后，该研究揭示了一种新的蒽生物降解途径，并为纳米辅助细菌修复提供了新的视角。
