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最新的快速现场检测食品中真菌毒素的策略:综述。

Latest strategies for rapid and point of care detection of mycotoxins in food: A review.

机构信息

College of Food Science and Technology, Henan Key Laboratory of Cereal and Oil Food Safety Inspection and Control, Henan University of Technology, Zhengzhou, 450001, China.

College of Food Science and Technology, Henan Key Laboratory of Cereal and Oil Food Safety Inspection and Control, Henan University of Technology, Zhengzhou, 450001, China.

出版信息

Anal Chim Acta. 2023 Mar 15;1246:340888. doi: 10.1016/j.aca.2023.340888. Epub 2023 Jan 24.

Abstract

Mycotoxins contaminated in agricultural products are often highly carcinogenic and genotoxic to humans. With the streamlining of the food industry chain and the improvement of food safety requirements, the traditional laboratory testing mode is constantly challenged due to the expensive equipment, complex operation steps, and lag in testing results. Therefore, rapid detection methods are urgently needed in the food safety system. This review focuses on the latest strategies that can achieve rapid and on-site testing, with particular attention to the nanomaterials integrated biosensors. To provide researchers with the latest trends and inspiration in the field of rapid detection, we summarize several strategies suitable for point of care testing (POCT) of mycotoxins, including enzyme-linked immunoassay (ELISA), lateral flow assay (LFA), fluorescence, electrochemistry, and colorimetry assay. POCT-based strategies are all developing towards intelligence and portability, especially when combined with smartphones, making it easier to read signals for intuitive access and analysis of test data. Detection performance of the devices has also improved considerably with the integration of biosensors and nanomaterials.

摘要

农产品中污染的真菌毒素对人类通常具有高度致癌性和遗传毒性。随着食品产业链的精简和食品安全要求的提高,由于昂贵的设备、复杂的操作步骤以及检测结果的滞后,传统的实验室检测模式不断受到挑战。因此,食品安全系统迫切需要快速检测方法。本综述重点介绍了可实现快速现场检测的最新策略,特别关注了集成纳米材料的生物传感器。为了为研究人员提供快速检测领域的最新趋势和灵感,我们总结了几种适合现场即时检测(POCT)的真菌毒素的策略,包括酶联免疫吸附测定(ELISA)、侧向流动测定(LFA)、荧光、电化学和比色测定。基于 POCT 的策略都在朝着智能化和便携化的方向发展,特别是与智能手机结合使用时,更容易读取信号,直观地访问和分析测试数据。通过将生物传感器和纳米材料集成,检测设备的检测性能也得到了显著提高。

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