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从谷子()中获得的转基因水稻在田间耐高盐胁迫。

from Foxtail Millet () Confers Transgenic Rice Tolerance to High Salt Stress in the Field.

机构信息

Key Laboratory of Sustainable Dryland Agriculture, College of Agriculture, Shanxi Agricultural University, Jinzhong 030801, China.

Institute of Crop Sciences, National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, China.

出版信息

Int J Mol Sci. 2022 Jan 11;23(2):756. doi: 10.3390/ijms23020756.

Abstract

Salt stress is a major threat to crop quality and yield. Most experiments on salt stress-related genes have been conducted at the laboratory or greenhouse scale. Consequently, there is a lack of research demonstrating the merit of exploring these genes in field crops. Here, we found that the R2R3-MYB transcription factor from foxtail millet is expressed mainly in the roots and is induced by various abiotic stressors such as salt, drought, low nitrogen, and abscisic acid. is tentatively localized to the nucleus and activates transcription. It enhances salt tolerance in transgenic rice at the germination and seedling stages. overexpression increased shoot height, grain yield, and salt tolerance in field- and salt pond-grown transgenic rice. overexpression promotes abscisic acid (ABA) accumulation in transgenic rice and upregulates the ABA synthesis gene and the ABA signal transduction pathway-related genes and . Thus, improves salt tolerance in transgenic rice by regulating ABA synthesis and signal transduction. Using rice heterologous expression analysis, the present study introduced a novel candidate gene for improving salt tolerance and increasing yield in crops grown in saline-alkali soil.

摘要

盐胁迫是影响作物品质和产量的主要因素。大多数与盐胁迫相关基因的相关实验都是在实验室或温室中进行的。因此,缺乏研究探索这些基因在大田作物中的应用价值。在这里,我们发现来自谷子的 R2R3-MYB 转录因子主要在根部表达,并受到各种非生物胁迫诱导,如盐、干旱、低氮和脱落酸。它被暂时定位于细胞核并激活转录。它增强了转基因水稻在萌发和幼苗阶段的耐盐性。过表达增加了转基因水稻在田间和盐田生长的株高、产量和耐盐性。过表达促进了转基因水稻中脱落酸(ABA)的积累,并上调了 ABA 合成基因 和 ABA 信号转导途径相关基因 和 。因此, 通过调节 ABA 的合成和信号转导来提高转基因水稻的耐盐性。通过水稻的异源表达分析,本研究为在盐碱地种植的作物提高耐盐性和产量提供了一个新的候选基因。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19ac/8775554/4e6ad96db2c3/ijms-23-00756-g001.jpg

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