Zhang Yun, Feng Xinyi, Bian Lijun, Zhang Yan, Li Qian, Xu Yemin, She Qiang, Yan Caiwang, Lu Guotao, Wu Jian, Xiao Weiming, Ding Yanbing, Deng Bin
Department of Gastroenterology, The Affiliated Hospital of Yangzhou University, No. 368 Hanjiang Middle Road, Yangzhou 225001, China.
Department of Emergency, The Affiliated Suqian Hospital of Xuzhou Medical University, Suqian 223800, China.
J Clin Med. 2023 Jan 19;12(3):816. doi: 10.3390/jcm12030816.
The antibiotic resistance of () is a common cause of treatment failure. Previous studies showed that resistance may be related to some characteristics of patients. This study intended to investigate the resistance of to five commonly used antibiotics and risk factors in Yangzhou, China.
We recruited the subjects who joined the endoscopic screening program organized by the Affiliated Hospital of Yangzhou University between April 2018 and September 2019 and endoscopists would take biopsy samples from the antrum and the corpus of the stomach. The antrum biopsy specimens were used to culture . Next, we extracted DNA from strains and performed the specific DNA amplification. Finally, we use gene chip technology to test the susceptibility to clarithromycin, levofloxacin, metronidazole, amoxicillin and tetracycline. Multivariate logistic analyses were also performed to determine the risk factors for antibiotic resistance of .
A total of 461 strains were finally collected. The resistance rate of to clarithromycin, levofloxacin, metronidazole, amoxicillin and tetracycline was 41.0%, 44.9%, 38.8%, 6.3% and 1.1%, respectively. In addition, 16 multi-resistance patterns were detected, and strains resistant to all five antibiotics were not found. Multivariate analysis showed that past medical history and clinical outcomes were significantly associated with the resistance to clarithromycin. Drinking, gastrointestinal symptoms and a family history of gastric cancer were significantly associated with the resistance of to levofloxacin. Especially gastrointestinal symptoms were significantly associated with the resistance of to any antibiotic.
The resistance rates of to clarithromycin, levofloxacin and metronidazole were very high in Yangzhou, China, various factors were related to bacterial resistance, and grasping these influencing factors can guide treatment.
（某菌）的抗生素耐药性是治疗失败的常见原因。先前的研究表明，（该菌）耐药性可能与患者的某些特征有关。本研究旨在调查中国扬州地区（该菌）对五种常用抗生素的耐药性及危险因素。
我们招募了2018年4月至2019年9月期间参加扬州大学附属医院组织的内镜筛查项目的受试者，内镜医师从胃窦和胃体取活检样本。胃窦活检标本用于培养（该菌）。接下来，我们从（该菌）菌株中提取DNA并进行特异性DNA扩增。最后，我们使用基因芯片技术检测对克拉霉素、左氧氟沙星、甲硝唑、阿莫西林和四环素的敏感性。还进行了多因素逻辑分析以确定（该菌）抗生素耐药性的危险因素。
最终共收集到461株（该菌）菌株。（该菌）对克拉霉素、左氧氟沙星、甲硝唑、阿莫西林和四环素的耐药率分别为41.0%、44.9%、38.8%、6.3%和1.1%。此外，检测到16种多重耐药模式，未发现对所有五种抗生素均耐药的菌株。多因素分析表明，既往病史和临床结局与对克拉霉素的耐药性显著相关。饮酒、胃肠道症状和胃癌家族史与（该菌）对左氧氟沙星的耐药性显著相关。尤其是胃肠道症状与（该菌）对任何抗生素的耐药性显著相关。
在中国扬州地区，（该菌）对克拉霉素、左氧氟沙星和甲硝唑的耐药率非常高，多种因素与细菌耐药性有关，掌握这些影响因素可指导治疗。
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