Tuna Taskin, Wein Martin, Altmann Brigitte, Steinberg Thorsten, Fischer Jens, Att Wael
Department of Prosthodontics and Biomaterials, School of Dentistry, RWTH University Aachen, Pauwelsstr. 30, 52062 Aachen, Germany.
Division of Oral Biotechnology, Center for Dental Medicine, Medical Center-University of Freiburg, Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany.
Materials (Basel). 2023 Jan 20;16(3):961. doi: 10.3390/ma16030961.
The aim of this in vitro study was to investigate the effect of hydrogen peroxide (HO) on the surface properties of various zirconia-based dental implant materials and the response of human alveolar bone osteoblasts. For this purpose, discs of two zirconia-based materials with smooth and roughened surfaces were immersed in 20% HO for two hours. Scanning electron and atomic force microscopy showed no topographic changes after HO-treatment. Contact angle measurements (1), X-ray photoelectron spectroscopy (2) and X-ray diffraction (3) indicated that HO-treated surfaces (1) increased in hydrophilicity ( < 0.05) and (2) on three surfaces the carbon content decreased (33-60%), while (3) the monoclinic phase increased on all surfaces. Immunofluorescence analysis of the cell area and DNA-quantification and alkaline phosphatase activity revealed no effect of HO-treatment on cell behavior. Proliferation activity was significantly higher on three of the four untreated surfaces, especially on the smooth surfaces ( < 0.05). Within the limitations of this study, it can be concluded that exposure of zirconia surfaces to 20% HO for 2 h increases the wettability of the surfaces, but also seems to increase the monoclinic phase, especially on roughened surfaces, which can be considered detrimental to material stability. Moreover, the HO-treatment has no influence on osteoblast behavior.
本体外研究的目的是调查过氧化氢（HO）对各种氧化锆基牙科植入材料表面特性的影响以及对人牙槽骨成骨细胞的反应。为此，将具有光滑和粗糙表面的两种氧化锆基材料圆盘浸入20%的HO中两小时。扫描电子显微镜和原子力显微镜显示HO处理后表面形貌无变化。接触角测量（1）、X射线光电子能谱（2）和X射线衍射（3）表明，HO处理过的表面（1）亲水性增加（<0.05），且（2）在三个表面上碳含量下降（33 - 60%），而（3）所有表面上的单斜相均增加。对细胞面积、DNA定量和碱性磷酸酶活性的免疫荧光分析显示HO处理对细胞行为无影响。在四个未处理表面中的三个表面上增殖活性显著更高，尤其是在光滑表面上（<0.05）。在本研究的局限性范围内，可以得出结论，将氧化锆表面暴露于20%的HO中2小时会增加表面的润湿性，但似乎也会增加单斜相，尤其是在粗糙表面上，这可能被认为对材料稳定性不利。此外，HO处理对成骨细胞行为没有影响。
