Faculty of Pharmacy, Saint Petersburg State Chemical Pharmaceutical University, 14, Prof. Popov Str., 197376 Saint-Petersburg, Russia.
K.A. Tymyryazev Institute of Plant Physiology, Russian Academy of Sciences, 35, ul. Botanicheskaya, 127276 Moscow, Russia.
Nutrients. 2021 Oct 26;13(11):3811. doi: 10.3390/nu13113811.
In the present study, we explored the therapeutic potential of bioreactor-grown cell cultures of the medicinal plant species , and to treat carbohydrate metabolism disorders (CMDs) in laboratory rats. In the adrenaline model of hyperglycemia, aqueous suspensions of cell biomass pre-administered at a dose of 100 mg dry biomass/kg significantly reduced glucose level in animal blood 1-2.5 h ( and ) or 1 h () after adrenaline hydrochloride administration. In a streptozotocin-induced model of type 2 diabetes mellitus, the cell biomass of and acted towards normalization of carbohydrate and lipid metabolism, as evidenced by a significant reduction of daily diuresis (by 39-57%), blood-glucose level (by 46-51%), blood content in urine (by 78-80%) and total cholesterol (25-36%) compared to animals without treatment. Bioactive secondary metabolites identified in the cell cultures and potentially responsible for their actions were deltoside, 25(S)-protodioscin and protodioscin in ; furostanol-type steroidal glycosides and quinic acid derivatives in ; and ginsenosides and malonyl-ginsenosides in These results evidenced for high potential of bioreactor-grown cell suspensions of these species for prevention and treatment of CMD, which requires further investigation.
在本研究中，我们探索了药用植物 和 的生物反应器培养细胞在治疗实验室大鼠碳水化合物代谢紊乱（CMD）方面的潜力。在肾上腺素诱导的高血糖模型中，100mg 干生物质/千克剂量的细胞生物质水混悬液在肾上腺素盐酸盐给药后 1-2.5 小时（和）或 1 小时（）显著降低了动物血液中的葡萄糖水平。在链脲佐菌素诱导的 2 型糖尿病模型中， 和 的细胞生物质作用于碳水化合物和脂质代谢的正常化，这表现为每日尿量（减少 39-57%）、血糖水平（减少 46-51%）、尿液中血液含量（减少 78-80%）和总胆固醇（减少 25-36%）显著降低。在细胞培养物中鉴定出的生物活性次生代谢物，可能是其作用的原因，包括 在德尔塔皂甙、25(S)-原薯蓣皂甙和原薯蓣皂甙； 在呋甾烷醇型甾体糖苷和奎尼酸衍生物； 和 在人参皂甙和丙二酰基人参皂甙。这些结果证明了这些物种的生物反应器培养细胞悬浮液在预防和治疗 CMD 方面具有很高的潜力，这需要进一步研究。
