Mishra Snehasis, das Kaustav, Chatterjee Sujan, Sahoo Panchanan, Kundu Sudip, Pal Mrinal, Bhaumik Asim, Ghosh Chandan Kumar
School of Material Science and Nanotechnology, Jadavpur University, Kolkata700032, India.
Molecular Biology and Tissue Culture Laboratory, Post Graduate Department of Zoology, Vidyasagar College, Kolkata700006, India.
ACS Omega. 2023 Jan 27;8(5):4566-4577. doi: 10.1021/acsomega.2c04964. eCollection 2023 Feb 7.
Carbon dots (CQDs) have been widely investigated as prime candidates for developing a tumor theranostic platform due to their tunable fluorescence emission and excitation, high water solubility, good photostability, and biocompatibility. Among the CQDs, natural CQDs are an emerging class of nanomaterials in the carbon family. Herein, highly fluorescent carbon quantum dots (CQDs) were synthesized from orange juice using a one-step hydrothermal method and characterized by different techniques. After that, CQD/Ag heterostructures were synthesized by the reduction of silver salt, in particular silver nitrate (AgNO) solution using sodium borohydride (NaBH) in different ratios. The photostability and characterization of CQD/Ag heterostructures were investigated. At last, a comparative cellular toxicity measurement was done to select the superior CQD/Ag heterostructure in the human colorectal carcinoma (HCT 116) cell line along with the imaging property. The detailed cell death signaling was also observed in the HCT 116 cell line via the ROS-dependent mitochondrial-mediated pathway, where Akt (RAC-α serine/threonine-protein kinase) played a important role.
碳点（CQDs）因其可调节的荧光发射和激发、高水溶性、良好的光稳定性和生物相容性，已被广泛研究作为开发肿瘤诊疗平台的主要候选材料。在碳点中，天然碳点是碳家族中一类新兴的纳米材料。在此，采用一步水热法从橙汁中合成了高荧光碳量子点（CQDs），并通过不同技术对其进行了表征。之后，通过用硼氢化钠（NaBH）以不同比例还原银盐，特别是硝酸银（AgNO）溶液，合成了CQD/Ag异质结构。研究了CQD/Ag异质结构的光稳定性和表征。最后，进行了细胞毒性比较测量，以选择在人结肠直肠癌（HCT 116）细胞系中具有优异成像特性的CQD/Ag异质结构。还通过ROS依赖性线粒体介导途径在HCT 116细胞系中观察到了详细的细胞死亡信号传导，其中Akt（RAC-α丝氨酸/苏氨酸蛋白激酶）发挥了重要作用。
